Recombinant Mouse CD28 protein (Fc Chimera) (ab207114)

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Description

  • Product name

    Recombinant Mouse CD28 protein (Fc Chimera)
    See all CD28 proteins and peptides

  • Purity

    > 90 % SDS-PAGE.

  • Endotoxin level

    < 1.000 Eu/µg

  • Expression system

    HEK 293 cells

  • Accession

    P31041

  • Protein length

    Protein fragment

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Mouse

    • Sequence

      NKILVKQSPLLVVDSNEVSLSCRYSYNLLAKEFRASLYKGVNSDVEVCVG NGNFTYQPQFRSNAEFNCDGDFDNETVTFRLWNLHVNHTDIYFCKIEFMY PPPYLDNERSNGTIIHIKEKHLCHTQSSPK

    • Predicted molecular weight

      42 kDa including tags

    • Amino acids

      20 to 149

    • Additional sequence information

      Fused with a Human IgG1 Fc tag at the C-terminus (NP_031668.3).

Specifications

Our Abpromise guarantee covers the use of ab207114 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).

    pH: 7.50
    Constituents: 0.75% Glycine, 0.61% Tris, 5% Trehalose, Sodium chloride, L-Arginine

    Lyophilized from 0.22 µm filtered solution.

  • Reconstitution
    Reconstitute the lyophilized product in sterile deionized water to a final concentration of 1 mg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.

General Info

  • Alternative names

    • CD 28
    • CD28
    • CD28 antigen
    • CD28 molecule
    • CD28_HUMAN
    • MGC138290
    • T cell antigen CD28
    • T cell specific surface glycoprotein
    • T cell specific surface glycoprotein CD28
    • T-cell-specific surface glycoprotein CD28
    • TP44
    see all

  • Function

    Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival.

  • Tissue specificity

    Expressed in T-cells and plasma cells, but not in less mature B-cells.

  • Sequence similarities

    Contains 1 Ig-like V-type (immunoglobulin-like) domain.

  • Cellular localization

    Membrane.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant Mouse CD28 protein (Fc Chimera) (ab207114)
    SDS-PAGE - Recombinant Mouse CD28 protein (Fc Chimera) (ab207114)

    DTT-reduced SDS-PAGE analysis of ab207114 stained overnight with Coomassie Blue.

    DTT-reduced protein migrates as 55-60 kDa in SDS-PAGE due to glycosylation.

References

ab207114 has not yet been referenced specifically in any publications.

Publishing research using ab207114? Please let us know so that we can cite the reference in this datasheet.

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