• Nature
  • Source
    HEK 293 cells
  • Amino Acid Sequence
    • Accession
    • Species
    • Sequence
    • Molecular weight
      36 kDa including tags
    • Amino acids
      23 to 108
    • Tags
      Fc tag C-Terminus
    • Additional sequence information
      Fused with a human IgG1 Fc tag at the C-terminus.


Our Abpromise guarantee covers the use of ab220543 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications


  • Endotoxin level
    < 1.000 Eu/µg
  • Purity
    >95% by SDS-PAGE .

  • Form
  • Additional notes

     No activity loss was observed after storage at 4-8? for 12 months in lyophilized state; -70? for 3 months under sterile conditions after reconstitution.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at 4°C (stable for up to 12 months). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 7.50
    Constituents: 0.61% Tris, 5% Trehalose, 0.75% Glycine

  • Reconstitution
    It is recommended to reconstitute the lyophilized product in sterile deionized water to a final concentration of 1 mg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.

General Info

  • Alternative names
    • 4930549J05Rik
    • A430104F18Rik
    • AW552088
    • Cd247
    • CD247 antigen
    • CD247 antigen, zeta subunit
    • CD247 molecule
    • CD3
    • CD3 antigen, delta subunit
    • CD3 delta
    • CD3 epsilon
    • CD3 eta
    • CD3 gamma
    • CD3 molecule delta polypeptide
    • CD3 molecule, epsilon polypeptide
    • CD3 molecule, gamma polypeptide
    • CD3 zeta
    • CD3-DELTA
    • CD3d
    • CD3D antigen delta polypeptide
    • CD3d antigen, delta polypeptide (TiT3 complex)
    • CD3d molecule delta
    • CD3d molecule delta CD3 TCR complex
    • CD3d molecule, delta (CD3-TCR complex)
    • CD3D_HUMAN
    • CD3E
    • CD3e antigen
    • CD3E antigen epsilon polypeptide
    • CD3e antigen, epsilon polypeptide (TiT3 complex)
    • CD3e molecule epsilon
    • CD3e molecule epsilon CD3 TCR complex
    • CD3e molecule, epsilon (CD3-TCR complex)
    • CD3epsilon
    • CD3G
    • CD3g antigen
    • CD3G antigen gamma polypeptide
    • CD3g antigen, gamma polypeptide (TiT3 complex)
    • CD3g molecule gamma
    • CD3g molecule gamma CD3 TCR complex
    • CD3g molecule, gamma (CD3-TCR complex)
    • CD3H
    • CD3Q
    • CD3Z
    • CD3zeta
    • Ctg3
    • FLJ17620
    • FLJ17664
    • FLJ18683
    • FLJ79544
    • FLJ94613
    • IMD19
    • Leu-4
    • MGC138597
    • OKT3, delta chain
    • OTTHUMP00000032544
    • T cell receptor
    • T cell receptor T3 delta chain
    • T cell receptor T3 gamma chain
    • T cell receptor T3 zeta chain
    • T cell receptor zeta chain
    • T cell surface glycoprotein CD3
    • T cell surface glycoprotein CD3 delta chain
    • T cell surface glycoprotein CD3 epsilon chain
    • T cell surface glycoprotein CD3 gamma chain
    • T cell surface glycoprotein CD3 zeta chain
    • T-cell antigen receptor complex, delta subunit of T3
    • T-cell antigen receptor complex, epsilon subunit of T3
    • T-cell antigen receptor complex, gamma subunit of T3
    • T-cell antigen receptor complex, zeta subunit of CD3
    • T-cell receptor T3 delta chain
    • T-cell receptor T3 gamma chain
    • T-cell surface antigen T3/Leu-4 epsilon chain
    • T-cell surface glycoprotein CD3 delta chain
    • T-cell surface glycoprotein CD3 gamma chain
    • T3
    • T3d
    • T3e
    • T3g
    • T3z
    • TCRE
    • TCRk
    • Tcrz
    • TCRzeta
    see all
  • Function
    The CD3 complex mediates signal transduction.
  • Involvement in disease
    Defects in CD3D are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
  • Sequence similarities
    Contains 1 ITAM domain.
  • Cellular localization
  • Information by UniProt


  • SDS-PAGE analysis of reduced ab220543 stained overnight with Coomassie Blue.

    The reducing protein migrates as 45 kDa in SDS-PAGE.


ab220543 has not yet been referenced specifically in any publications.

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