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    recombinant-mouse-cleaved-caspase-3-protein-ab52071.pdf

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Recombinant mouse Cleaved Caspase-3 protein (ab52071)

  • Datasheet
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Key features and details

  • Expression system: Escherichia coli
  • Active: Yes

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Description

  • Product name

    Recombinant mouse Cleaved Caspase-3 protein
    See all Cleaved Caspase-3 proteins and peptides
  • Biological activity

    SPECIFIC ACTIVITY: 300,000 units/mg. One unit of the recombinant caspase-3 is the enzyme activity that cleaves 1 nmol of the caspase substrate DEVD-pNA (pNA: pnitroanaline) per hour at 37°C.

  • Expression system

    Escherichia coli
  • Accession

    P42574
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Mouse

Associated products

    Specifications

    Our Abpromise guarantee covers the use of ab52071 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Form

      Lyophilized
    • Additional notes

      Cleaved caspase-3 is useful in studying enzyme regulation, determining target substrates, screening caspase inhibitors, or as a positive control in caspase activity assays. We recommend using 1 unit/assay for analyzing caspase activity.

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

      pH: 7.20
      Constituents: 0.154% DTT, 1.19% HEPES, 0.292% EDTA, 5% Glycerol, 0.29% Sodium chloride, 0.1% CHAPS

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    • Reconstitution
      Reconstitute to 1 unit per µl in PBS containing 15% glycerol.

    General Info

    • Alternative names

      • active caspase 3
      • Apopain
      • CASP 3
      • CASP-3
      • CASP3
      • CASP3_HUMAN
      • Caspase 3
      • Caspase-3 subunit p12
      • CPP 32
      • CPP-32
      • CPP32B
      • Cysteine protease CPP32
      • PARP cleavage protease
      • Protein Yama
      • SCA-1
      • SCA1
      • SREBP cleavage activity 1
      • Yama
      see all
    • Function

      Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
      -Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.
    • Tissue specificity

      Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
    • Sequence similarities

      Belongs to the peptidase C14A family.
    • Post-translational
      modifications

      Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.
      S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.
    • Cellular localization

      Cytoplasm.
    • Target information above from: UniProt accession P42574 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab52071? Please let us know so that we can cite the reference in this datasheet.

    ab52071 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Question

    Hi.

    I'm interested in use one of your products as a positive control of active Casp3 (17kDa) by western blot. But I have doubts that I can use it. The webpage says "full length protein", but also "active" and the active form of Casp3 is 32kDa, so I don't know if its a mixture or what form it is. Can you help me with that? If I use this product (ab52071) in a western blot, what band/s I will find?

    Thank you very much.

    Read More

    Abcam community

    Verified customer

    Asked on Dec 06 2012

    Answer

    Thank you for contacting us.

    The "active" means this protein will act as an enzyme. In WB the protein will be observed at 25-30 kDa.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

    Read More

    Abcam Scientific Support

    Answered on Dec 06 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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