Recombinant Mouse CXCL10/IP-10/CRG-2 Protein (ab283918)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% HPLC
- Endotoxin level: <= 0.005 Eu/µg
- Active: Yes
- Suitable for: SDS-PAGE, HPLC, MS
Description
-
Product name
Recombinant Mouse CXCL10/IP-10/CRG-2 Protein
See all IP10 proteins and peptides -
Biological activity
Fully biologically active determined by dose dependent cell migration of human KHYG-1 cells. ED50 for this effect is ≤3.5ng/ml corresponding to specific activity of 2.85x105 units/mg.
-
Purity
> 95 % HPLC. -
Endotoxin level
<=0.005 Eu/µg -
Expression system
HEK 293 cells -
Accession
-
Protein length
Protein fragment -
Animal free
Yes -
Carrier free
Yes -
Nature
Recombinant -
-
Species
Mouse -
Sequence
IPLARTVRCNCIHIDDGPVRMRAIGKLEIIPASLSCPRVEIIATMKKNDE QRCLNPESKTIKNLMKAFSQKRSKRAP -
Predicted molecular weight
9 kDa -
Amino acids
22 to 98 -
Additional sequence information
N-terminal Glycine.
-
Specifications
Our Abpromise guarantee covers the use of ab283918 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
Applications
SDS-PAGE
HPLC
Mass Spectrometry
-
Form
Lyophilized -
Concentration information loading...
Preparation and Storage
-
Stability and Storage
Shipped at Room Temperature. Store at Room Temperature.
pH: 7.40
Constituents: 0.248% Monobasic dihydrogen potassium phosphate, 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphateThis product is an active protein and may elicit a biological response in vivo, handle with caution.
-
ReconstitutionStore lyophilized form at room temperature. Reconstitute in PBS, aliquot and store at -80°C for 12 months or +4°C for 1 week. Avoid repeated freeze-thaw. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.
General Info
-
Alternative names
- Interferon gamma induced factor MOB1, mouse, homolog of
- Interferon gamma induced protein 10
- 10 kDa interferon gamma induced protein
see all -
Function
Chemotactic for monocytes and T-lymphocytes. Binds to CXCR3. -
Sequence similarities
Belongs to the intercrine alpha (chemokine CxC) family. -
Post-translational
modificationsCXCL10(1-73) is produced by proteolytic cleavage after secretion from keratinocytes. -
Cellular localization
Secreted. - Information by UniProt
Images
-
Functional Studies - Recombinant Mouse CXCL10/IP-10/CRG-2 Protein (ab283918)
Fully biologically active determined by dose dependent cell migration of human KHYG-1 cells. ED50 for this effect is ≤3.5ng/ml corresponding to specific activity of 2.85x105 units/mg.
Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.
Lot GR3415974 -2
-
SDS-PAGE - Recombinant Mouse CXCL10/IP-10/CRG-2 Protein (ab283918)SDS-page analysis of ab283918
-
Mass Spectrometry - Recombinant Mouse CXCL10/IP-10/CRG-2 Protein (ab283918)Mass determination by ESI-TOF.
Predicted MW is 8758.43 Da. (+/- 10 Da by ESI-TOF). Observed MW is 8757.00 Da.
Significant heterogeneity in the protein due to multiple glycoforms.
-
HPLC - Recombinant Mouse CXCL10/IP-10/CRG-2 Protein (ab283918)HPLC analysis of ab283918
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
References (0)
ab283918 has not yet been referenced specifically in any publications.
