Recombinant mouse Cyclophilin 40 protein (Active) (ab229174)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies, MS
Description
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Product name
Recombinant mouse Cyclophilin 40 protein (Active)
See all Cyclophilin 40 proteins and peptides -
Biological activity
Specific activity is > 700 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1 nmole of suc-AAFP-PNA per minute at 37°C in Tris–HCl pH 8.0 using chymotrypsin.
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Purity
> 90 % SDS-PAGE.
ab229174 was purified using conventional chromatography techniques. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Mouse -
Sequence
MGSSHHHHHHSSGLVPRGSHMGSEFMSHASPAAKPSNSKNPRVFFDVDIG GERVGRIVLELFADIVPKTAENFRALCTGEKGTGSTTGKPLHFKGCPFHR IIKKFMIQGGDFSNQNGTGGESIYGEKFEDENFHYKHDREGLLSMANAGP NTNGSQFFITTVPTPHLDGKHVVFGQVIKGLGVARTLENVEVNGEKPAKL CVIAECGELKEGDDWGIFPKDGSGDSHPDFPEDADIDLKDVDKILLISED LKNIGNTFFKSQNWEMAIKKYAKVLRYVDSSKAVIEKADRSRLQPIALSC VLNIGACKLKMSNWQGAIDSCLEALEMDPSNTKALYRKAQGWQGLKEYDQ ALADLKKAQEIAPGDKAIQAELLKVKQMIKAQKDKEKAVYAKMFA -
Predicted molecular weight
43 kDa including tags -
Amino acids
1 to 370 -
Tags
His tag N-Terminus -
Additional sequence information
NP_080628.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab229174 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
Mass Spectrometry
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Mass spectrometry
MALDI-TOF -
Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.32% Tris HCl, 10% Glycerol (glycerin, glycerine), 0.02% DTTThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- 40 kDa peptidyl prolyl cis trans isomerase
- 40 kDa peptidyl prolyl cis trans isomerase D
- 40 kDa peptidyl-prolyl cis-trans isomerase
see all -
Function
PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. -
Tissue specificity
Widely expressed. -
Sequence similarities
Belongs to the cyclophilin-type PPIase family. PPIase D subfamily.
Contains 1 PPIase cyclophilin-type domain.
Contains 3 TPR repeats. -
Cellular localization
Cytoplasm. - Information by UniProt
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab229174 has not yet been referenced specifically in any publications.