Recombinant Mouse DLK-1 protein (Fc fragment) (ab108574)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 90% SDS-PAGE
- Endotoxin level: < 0.100 Eu/µg
- Suitable for: Functional Studies, SDS-PAGE
Description
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Product name
Recombinant Mouse DLK-1 protein (Fc fragment)
See all DLK-1 proteins and peptides -
Biological activity
Inhibits adipogenesis of 3T3L-1 cells. Induces Sox-9 in 3T3L-1 cells. -
Purity
> 90 % SDS-PAGE.
ab108574 is 0.2µm filtered -
Endotoxin level
< 0.100 Eu/µg -
Expression system
HEK 293 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Mouse -
Predicted molecular weight
75 kDa -
Amino acids
1 to 305 -
Additional sequence information
Fused with the Fc region of Human IgG1 at the C-terminus.
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Specifications
Our Abpromise guarantee covers the use of ab108574 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
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Form
Lyophilized -
Additional notes
Working aliquots are stable for up to 3 months when stored at -20°C. Inhibits adipogenesis of 3T3L-1 cells. Induces Sox-9 in 3T3L-1 cells.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituent: PBS
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ReconstitutionReconstitute with 100µl sterile water.
General Info
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Alternative names
- Adipocyte differentiation inhibitor protein
- Brevideltin
- Delta like 1 homolog
see all -
Function
May have a role in neuroendocrine differentiation. -
Tissue specificity
Found within the stromal cells in close contact to the vascular structure of placental villi, yolk sac, fetal liver, adrenal cortex and pancreas and in the beta cells of the islets of Langerhans in the adult pancreas. Found also in some forms of neuroendocrine lung tumor tissue. -
Sequence similarities
Contains 6 EGF-like domains. -
Post-translational
modificationsN- and O-glycosylated. -
Cellular localization
Membrane. - Information by UniProt
Images
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Induction of SOX-9 with the treatment of recombinant mouse DLK-1-Fc (ab108574).
A mouse preadpipocyte cell line, 3T3L1, was stimulated with 5µg/ml of ab108574 as in indicated time points and each cell lysate was prepared and subjected to western blot by using anti-rabbit Sox-9 and anti-rabbit GAPDH.1=0 min; 2=10 min; 3=30 min; 4=1 h; 5=2 h; 6=3 h; 7=4 h; 8=24 h.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab108574 has not yet been referenced specifically in any publications.