Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 75% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies, WB
Product nameRecombinant mouse DRAK2 protein (Active)
See all DRAK2 proteins and peptides
Specific Activity: 5 pmole/min/μg
Assay Conditions: Reaction was performed in 50 mM Tris pH 7.4, 5 mM MgCl2, 1 mM DTT, 20 μM ATP, 0.2 mg/ml MRCL3 Peptide. Reaction was initiated with the addition of Mouse DRAK2 and was incubated at 30°C for 45 min. The luminescence was read with the Kinase Glo Max reagent.
Purity>= 75 % SDS-PAGE.
Expression systemBaculovirus infected Sf9 cells
Protein lengthProtein fragment
SequenceSRRRFDCRSV SGLLTTTPQT PIKTENFNNF YTLTPKELGR GKFAVVRQCI SKSTGQEYAA KSLKKRRRGQ DCRAEILHEI AVLELARSCP HVINLHEVYE NATEIILVLE YAAGGEIFNL CLPELAEMVS ENDVIRLIKQ ILEGVHYLHQ NNIVHLDLKP QNILLSSIYP LGDIKIVDFG MSRKIGNASE LREIMGTPEY LAPEILNYDP ITTATDMWNI GIIAYMLLTH TSPFVGEDNQ ETYLNISQVN VDYSEEMFSS VSQLATDFIQ SLLVKNPEKR PTAESCLSHS WLQQWDFGSL FHPEETSGSS QIQDLTLRSS EEKTSKSSCN GSCGAREDKE NIPEDGSLVS KRFRFDDSLP SPHELVPDLF C
Predicted molecular weight68 kDa including tags
Amino acids2 to 372
TagsGST tag N-Terminus
Our Abpromise guarantee covers the use of ab271491 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Store at -80°C. Do Not Freeze.
Constituents: 1.19% HEPES, 0.001% Polyoxyethylene lauryl ether, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.13% Magnesium chloride, 1.13% Potassium chloride, 5% Glycerol (glycerin, glycerine), 0.003% EDTA, 0.03% Glutathione
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- DAP kinase related apoptosis inducing protein
- DAP kinase related apoptosis inducing protein kinase 2
- DAP kinase-related apoptosis-inducing protein kinase 2
FunctionActs as a positive regulator of apoptosis.
Tissue specificityHighly expressed in placenta, lung, pancreas. Lower levels in heart, brain, liver, skeletal muscle and kidney.
Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.
Contains 1 protein kinase domain.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab271491 has not yet been referenced specifically in any publications.