Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Endotoxin level: < 0.100 Eu/µg
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
Product nameRecombinant mouse EBI3 protein
See all EBI3 proteins and peptides
Purity> 90 % SDS-PAGE.
Endotoxin level< 0.100 Eu/µg
Expression systemEscherichia coli
Protein lengthProtein fragment
SequenceMALVALSQPR VQCHASRYPV AVDCSWTPLQ APNSTRSTSF IATYRLGVAT QQQSQPCLQR SPQASRCTIP DVHLFSTVPY MLNVTAVHPG GASSSLLAFV AERIIKPDPP EGVRLRTAGQ RLQVLWHPPA SWPFPDIFSL KYRLRYRRRG ASHFRQVGPI EATTFTLRNS KPHAKYCIQV SAQDLTDYGK PSDWSLPGQV ESAPHKP
Predicted molecular weight23 kDa
Amino acids23 to 228
Our Abpromise guarantee covers the use of ab206301 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Assay data for recombinant Mouse EBI3 is based upon qualitative binding to anti-EBI3 antibody.
Centrifuge vial before opening. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 - 0.5 mg/ml, which can be further diluted into other aqueous solutions.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -20°C. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA). Please see notes section.
Constituent: 0.26% Sodium citrate
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionCentrifuge vial before opening. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 - 0.5 mg/ml, which can be further diluted into other aqueous solutions.
- cytokine receptor
- EBV induced gene 3 protein
FunctionAssociates with IL27 to form the IL-27 interleukin, a heterodimeric cytokine which functions in innate immunity. IL-27 has pro- and anti-inflammatory properties, that can regulate T-helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T-helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon-gamma/IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1/TCCR. Another important role of IL-27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines.
Sequence similaritiesBelongs to the type I cytokine receptor family. Type 3 subfamily.
Contains 2 fibronectin type-III domains.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab206301 has been referenced in 1 publication.
- Ma N et al. Ebi3 promotes T- and B-cell division and differentiation via STAT3. Mol Immunol 107:61-70 (2019). PubMed: 30660991