Recombinant mouse EGF protein (ab72994)

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Description

  • Product name

    Recombinant mouse EGF protein
    See all EGF proteins and peptides

  • Biological activity

    The ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells (measured by 3H-thymidine uptake) is < 0.1 ng/ml.

  • Purity

    > 95 % SDS-PAGE.
    Purity is greater than 97% as determined by reducing and non-reducing SDS-PAGE and analytical HPLC.

  • Expression system

    Escherichia coli

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Mouse

    • Sequence

      NSYPGCPSSY DGYCLNGGVC MHIESLDSYT CNCVIGYSGD RCQTRDLRWW ELR

Associated products

Specifications

Our Abpromise guarantee covers the use of ab72994 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

  • Form

    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Reconstitute with sterile water at a concentration of 0.1-0.5 mg/ml, which can be further diluted into other aqueous solutions. Reconstituted material should be aliquoted and frozen at -20oC. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.

General Info

  • Alternative names

    • Beta urogastrone
    • beta-urogastrone
    • EGF
    • EGF_HUMAN
    • Epidermal growth factor
    • HOMG4
    • OTTHUMP00000219721
    • OTTHUMP00000219722
    • Pro epidermal growth factor
    • URG
    • Urogastrone
    see all

  • Function

    EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941).

  • Tissue specificity

    Expressed in kidney, salivary gland, cerebrum and prostate.

  • Involvement in disease

    Hypomagnesemia 4

  • Sequence similarities

    Contains 9 EGF-like domains.
    Contains 9 LDL-receptor class B repeats.

  • Post-translational
    modifications

    O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor).

  • Cellular localization

    Membrane.
  • Information by UniProt

Images

  • Other - Recombinant mouse EGF protein (ab72994)
    Other - Recombinant mouse EGF protein (ab72994)
    ab72994 used in Western Blot. Figure: 1 ug in each lane (-) non-reducing conditions and (+) reducing conditions in a 4-20% Tris-Glycine gel stained with Coomassie Blue. Mouse EGF is predicted to be a homodimer with a MW of 12.4 kDa.

References

ab72994 has not yet been referenced specifically in any publications.

Publishing research using ab72994? Please let us know so that we can cite the reference in this datasheet.

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