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    recombinant-mouse-egf-protein-ab72994.pdf

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Signal Transduction Growth Factors/Hormones EGF
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Recombinant mouse EGF protein (ab72994)

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Western blot - Recombinant mouse EGF protein (ab72994)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 95% SDS-PAGE
    • Active: Yes
    • Suitable for: SDS-PAGE, Functional Studies

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    Description

    • Product name

      Recombinant mouse EGF protein
      See all EGF proteins and peptides
    • Biological activity

      The ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells (measured by 3H-thymidine uptake) is < 0.1 ng/ml.
    • Purity

      > 95 % SDS-PAGE.
      Purity is greater than 97% as determined by reducing and non-reducing SDS-PAGE and analytical HPLC.
    • Expression system

      Escherichia coli
    • Protein length

      Full length protein
    • Animal free

      No
    • Nature

      Recombinant
      • Species

        Mouse
      • Sequence

        NSYPGCPSSY DGYCLNGGVC MHIESLDSYT CNCVIGYSGD RCQTRDLRWW ELR

    Associated products

      Specifications

      Our Abpromise guarantee covers the use of ab72994 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      • Applications

        SDS-PAGE

        Functional Studies

      • Form

        Lyophilized
      • Concentration information loading...

      Preparation and Storage

      • Stability and Storage

        Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

        This product is an active protein and may elicit a biological response in vivo, handle with caution.

      • Reconstitution
        Reconstitute with sterile water at a concentration of 0.1-0.5 mg/ml, which can be further diluted into other aqueous solutions. Reconstituted material should be aliquoted and frozen at -20oC. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.

      General Info

      • Alternative names

        • Beta urogastrone
        • beta-urogastrone
        • EGF
        • EGF_HUMAN
        • Epidermal growth factor
        • HOMG4
        • OTTHUMP00000219721
        • OTTHUMP00000219722
        • Pro epidermal growth factor
        • URG
        • Urogastrone
        see all
      • Function

        EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941).
      • Tissue specificity

        Expressed in kidney, salivary gland, cerebrum and prostate.
      • Involvement in disease

        Hypomagnesemia 4
      • Sequence similarities

        Contains 9 EGF-like domains.
        Contains 9 LDL-receptor class B repeats.
      • Post-translational
        modifications

        O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor).
      • Cellular localization

        Membrane.
      • Target information above from: UniProt accession P01133 The UniProt Consortium
        The Universal Protein Resource (UniProt) in 2010
        Nucleic Acids Res. 38:D142-D148 (2010) .

        Information by UniProt

      Images

      • Western blot - Recombinant mouse EGF protein (ab72994)
        Western blot - Recombinant mouse EGF protein (ab72994)
        ab72994 used in Western Blot. Figure: 1 ug in each lane (-) non-reducing conditions and (+) reducing conditions in a 4-20% Tris-Glycine gel stained with Coomassie Blue. Mouse EGF is predicted to be a homodimer with a MW of 12.4 kDa.

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab72994? Please let us know so that we can cite the reference in this datasheet.

      ab72994 has not yet been referenced specifically in any publications.

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      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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