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Signal Transduction Growth Factors/Hormones EGF
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Bioactive grade

Recombinant mouse EGF protein (Animal Free) (ab206643)

  • Datasheet
  • SDS
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SDS-PAGE - Recombinant mouse EGF protein (Animal Free) (ab206643)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 97% SDS-PAGE
    • Endotoxin level: < 0.050 Eu/µg
    • Active: Yes
    • Suitable for: Functional Studies, SDS-PAGE, HPLC

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    Description

    • Product name

      Recombinant mouse EGF protein (Animal Free)
      See all EGF proteins and peptides
    • Biological activity

      The activity is determined by the dose-dependent proliferation of mouse BALB/c 3T3 cells and is typically less than 0.1 ng/mL. This corresponds to an expected specific activity of 1 x 107 units/mg.

    • Purity

      > 97 % SDS-PAGE.
      Purity is greater than 97% as determined by reducing and non-reducing SDS-PAGE and analytical HPLC.
    • Endotoxin level

      < 0.050 Eu/µg
    • Expression system

      Escherichia coli
    • Accession

      P01132
    • Protein length

      Full length protein
    • Animal free

      Yes
    • Nature

      Recombinant
      • Species

        Mouse
      • Sequence

        NSYPGCPSSY DGYCLNGGVC MHIESLDSYT CNCVIGYSGD RCQTRDLRWW ELR
      • Predicted molecular weight

        6 kDa
      • Amino acids

        977 to 1029
      • Additional sequence information

        This product is for the mature full length protein. The signal peptide is not included.

    Associated products

      Specifications

      Our Abpromise guarantee covers the use of ab206643 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      • Applications

        Functional Studies

        SDS-PAGE

        HPLC

      • Form

        Lyophilized
      • Concentration information loading...

      Preparation and Storage

      • Stability and Storage

        Shipped at Room Temperature. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

        Constituent: 0.16% Sodium phosphate

        This product is an active protein and may elicit a biological response in vivo, handle with caution.

      • Reconstitution
        Reconstitute in sterile water at 0.1 mg/mL. Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.

      General Info

      • Alternative names

        • Beta urogastrone
        • beta-urogastrone
        • EGF
        • EGF_HUMAN
        • Epidermal growth factor
        • HOMG4
        • OTTHUMP00000219721
        • OTTHUMP00000219722
        • Pro epidermal growth factor
        • URG
        • Urogastrone
        see all
      • Function

        EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941).
      • Tissue specificity

        Expressed in kidney, salivary gland, cerebrum and prostate.
      • Involvement in disease

        Hypomagnesemia 4
      • Sequence similarities

        Contains 9 EGF-like domains.
        Contains 9 LDL-receptor class B repeats.
      • Post-translational
        modifications

        O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor).
      • Cellular localization

        Membrane.
      • Target information above from: UniProt accession P01133 The UniProt Consortium
        The Universal Protein Resource (UniProt) in 2010
        Nucleic Acids Res. 38:D142-D148 (2010) .

        Information by UniProt

      Images

      • SDS-PAGE - Recombinant mouse EGF protein (Animal Free) (ab206643)
        SDS-PAGE - Recombinant mouse EGF protein (Animal Free) (ab206643)

        SDS-PAGE analysis of ab216643 (1 μg) stained with Coomassie blue:

        Lane 1: Non-reduced

        Lane 2: Reduced

        4-20% Tris-Glycine gel

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (0)

      Publishing research using ab206643? Please let us know so that we can cite the reference in this datasheet.

      ab206643 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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