Description

  • Product name

    Recombinant Mouse eIF4E protein (His tag)
    See all eIF4E proteins and peptides
  • Purity

    > 95 % SDS-PAGE.
    ab222974 was purified by using conventional chromatography.
  • Expression system

    Escherichia coli
  • Accession

  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Mouse
    • Sequence

      MGSSHHHHHH SSGLVPRGSH MGSHMATVEP ETTPTTNPPP AEEEKTESNQ EVANPEHYIK HPLQNRWALW FFKNDKSKTW QANLRLISKF DTVEDFWALY NHIQLSSNLM PGCDYSLFKD GIEPMWEDEK NKRGGRWLIT LNKQQRRSDL DRFWLETLLC LIGESFDDYS DDVCGAVVNV RAKGDKIAIW TTECENRDAV THIGRVYKER LGLPPKIVIG YQSHADTATK SGSTTKNRFV V
    • Predicted molecular weight

      28 kDa including tags
    • Amino acids

      1 to 217
    • Tags

      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab222974 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Mass Spectrometry

    SDS-PAGE

  • Mass spectrometry

    MALDI-TOF
  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.0
    Constituents: 0.32% Tris HCl, 10% Glycerol, 0.0154% DTT, 0.58% Sodium chloride, 0.00174% PMSF

General Info

  • Alternative names

    • AUTS19
    • CBP
    • eIF 4E
    • EIF 4F
    • eIF 4F 25 kDa subunit
    • eIF-4E
    • eIF-4F 25 kDa subunit
    • eIF4E
    • EIF4E1
    • EIF4EL1
    • EIF4F
    • Eukaryotic translation initiation factor 4 E
    • Eukaryotic translation initiation factor 4E
    • Eukaryotic translation initiation factor 4E like 1
    • IF4E_HUMAN
    • Messanger RNA Cap Binding Protein eIF 4E
    • MGC111573
    • mRNA cap binding protein
    • mRNA cap-binding protein
    see all
  • Function

    Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (By similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.
  • Sequence similarities

    Belongs to the eukaryotic initiation factor 4E family.
  • Post-translational
    modifications

    Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex.
  • Information by UniProt

Images

  • 15% SDS-PAGE - Recombinant Mouse eIF4E protein (His tag) (3 µg ab222974).

     

References

ab222974 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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