Overview

Description

  • Nature

    Recombinant
  • Source

    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species

      Mouse
    • Sequence

      MQVTVQSSPN FTQHVREQSL VTDQLSRRLI RTYQLYSRTS GKHVQVLANK RINAMAEDGD PFAKLIVETD TFGSRVRVRG AETGLYICMN KKGKLIAKSN GKGKDCVFTE IVLENNYTAL QNAKYEGWYM AFTRKGRPRK GSKTRQHQRE VHFMKRLPRG HHTTEQSLRF EFLNYPPFTR SLRGSQRTWA PEPR
    • Molecular weight

      23 kDa
    • Amino acids

      23 to 215
    • Additional sequence information

      This is the mature protein without the signal peptide.

Specifications

Our Abpromise guarantee covers the use of ab218082 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    The activity is determined by its ability to induce proliferation of mouse 3T3 cells and is typically less than 20 ng/mL.  This corresponds to an expected specific activity of 5 x 104 units/mg.

  • Applications

    Functional Studies

    SDS-PAGE

  • Endotoxin level

    < 0.050 Eu/µg
  • Purity

    > 95 % SDS-PAGE.
    The purity of ab218082 is typically 97% as determined by reducing and non-reducing SDS-PAGE.
  • Form

    Lyophilised
  • Additional notes

    There is 100% homology between Human 1-194 aa and mouse Mouse 23-215 aa FGF-8. 

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at room temperature. Store at -20°C.

    pH: 7.50
    Constituents: 0.29% Sodium chloride, 0.08% Sodium phosphate

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Sterile water at 0.1 mg/mL. Centrifuge vial before opening. Suspend the product by gently pipetting sterile water down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80 ºC and avoid repeat freeze thaws.

General Info

  • Alternative names

    • AIGF
    • Androgen induced growth factor
    • Androgen-induced growth factor
    • FGF 8
    • FGF-8
    • FGF-8b
    • FGF8
    • FGF8_HUMAN
    • Fibroblast growth factor 8
    • Fibroblast growth factor 8 (androgen induced)
    • Fibroblast growth factor 8 precursor
    • HBGF 8
    • HBGF-8
    • HBGF8
    • Heparin-binding growth factor 8
    • HH6
    • KAL6
    see all
  • Function

    Stimulates growth of the cells in an autocrine manner. Mediates hormonal action on the growth of cancer cells.
  • Involvement in disease

    Defects in FGF8 are the cause of Kallmann syndrome type 6 (KAL6) [MIM:612702]. Kallmann syndrome is a disorder that associates hypogonadotropic hypogonadism and anosmia. Anosmia or hyposmia is related to the absence or hypoplasia of the olfactory bulbs and tracts. Hypogonadism is due to deficiency in gonadotropin-releasing hormone and probably results from a failure of embryonic migration of gonadotropin-releasing hormone-synthesizing neurons. In some patients other developmental anomalies can be present, which include renal agenesis, cleft lip and/or palate, selective tooth agenesis, and bimanual synkinesis. In some cases anosmia may be absent or inconspicuous.
    Defects in FGF8 are a cause of idiopathic hypogonadotropic hypogonadism (IHH) [MIM:146110]. IHH is defined as a deficiency of the pituitary secretion of follicle-stimulating hormone and luteinizing hormone, which results in the impairment of pubertal maturation and of reproductive function.
  • Sequence similarities

    Belongs to the heparin-binding growth factors family.
  • Developmental stage

    In adults expression is restricted to the gonads.
  • Cellular localization

    Secreted.
  • Information by UniProt

Images

  • SDS-PAGE analysis of ab218082 at 1 μg under (1) non-reducing and (2) reducing conditions in a 4-20% Tris-Glycine gel, stained with Coomassie Blue. 

References

ab218082 has not yet been referenced specifically in any publications.

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