Recombinant mouse FGF8 protein (Active) (ab218083)
- Datasheet
- References
- Protocols
Description
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Product name
Recombinant mouse FGF8 protein (Active)
See all FGF8 proteins and peptides -
Biological activity
The activity is determined by its ability to induce proliferation of mouse 3T3 cells and is typically less than 20 ng/mL. This corresponds to an expected specific activity of 5 x 104 units/mg.
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Purity
> 95 % SDS-PAGE.
The purity of ab218083 is typically 97% as determined by reducing and non-reducing SDS-PAGE. -
Endotoxin level
< 0.050 Eu/µg -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Mouse -
Sequence
MQVTVQSSPN FTQHVREQSL VTDQLSRRLI RTYQLYSRTS GKHVQVLANK RINAMAEDGD PFAKLIVETD TFGSRVRVRG AETGLYICMN KKGKLIAKSN GKGKDCVFTE IVLENNYTAL QNAKYEGWYM AFTRKGRPRK GSKTRQHQRE VHFMKRLPRG HHTTEQSLRF EFLNYPPFTR SLRGSQRTWA PEPR -
Predicted molecular weight
23 kDa -
Amino acids
23 to 215 -
Additional sequence information
Mature protein without the signal peptide. Sequence is from mouse isoform FGF-8B. There is 100% homology between human and mouse FGF-8.
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Specifications
Our Abpromise guarantee covers the use of ab218083 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
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Form
Lyophilised -
Additional notes
There is 100% homology between Human 1-194 aa and mouse Mouse 23-215 aa FGF-8.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at Room Temperature. Store at -20°C. Working aliquots stored with a carrier protein are stable for at least 3 months at -20°C to -80°C..
pH: 7.50
Constituents: 0.29% Sodium chloride, 0.08% Sodium phosphate
Lyophilized from a sterile filtered aqueous solution.This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionSterile water at 0.1 mg/mL. Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80 ºC and avoid repeat freeze thaws.
General Info
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Alternative names
- AIGF
- Androgen induced growth factor
- Androgen-induced growth factor
see all -
Function
Stimulates growth of the cells in an autocrine manner. Mediates hormonal action on the growth of cancer cells. -
Involvement in disease
Defects in FGF8 are the cause of Kallmann syndrome type 6 (KAL6) [MIM:612702]. Kallmann syndrome is a disorder that associates hypogonadotropic hypogonadism and anosmia. Anosmia or hyposmia is related to the absence or hypoplasia of the olfactory bulbs and tracts. Hypogonadism is due to deficiency in gonadotropin-releasing hormone and probably results from a failure of embryonic migration of gonadotropin-releasing hormone-synthesizing neurons. In some patients other developmental anomalies can be present, which include renal agenesis, cleft lip and/or palate, selective tooth agenesis, and bimanual synkinesis. In some cases anosmia may be absent or inconspicuous.
Defects in FGF8 are a cause of idiopathic hypogonadotropic hypogonadism (IHH) [MIM:146110]. IHH is defined as a deficiency of the pituitary secretion of follicle-stimulating hormone and luteinizing hormone, which results in the impairment of pubertal maturation and of reproductive function. -
Sequence similarities
Belongs to the heparin-binding growth factors family. -
Developmental stage
In adults expression is restricted to the gonads. -
Cellular localization
Secreted. - Information by UniProt
Images
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Functional Studies - Recombinant mouse FGF8 protein (Active) (ab218083)Functional analysis of ab218083 -
SDS-PAGE - Recombinant mouse FGF8 protein (Active) (ab218083)SDS PAGE analysis of ab218083 under non-reducing (-) and reducing (+) conditions. Stained with Coomassie Blue.
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Functional Studies - Recombinant mouse FGF8 protein (Active) (ab218083)Bioassay showing FGF8 induced proliferation of 3T3 cells using ab218083.
References
ab218083 has not yet been referenced specifically in any publications.
