Bioactive grade

Recombinant mouse G-CSF protein (Active) (ab243120)

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Description

  • Product name

    Recombinant mouse G-CSF protein (Active)
    See all G-CSF proteins and peptides

  • Biological activity

    Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine NFS 60 cells is less than 0.05 ng/ml, corresponding to a specific activity of >2.0x107 IU/mg.

  • Purity

    > 98 % SDS-PAGE.
    >98 % by HPLC.

  • Endotoxin level

    < 1.000 Eu/µg

  • Expression system

    Escherichia coli

  • Accession

    P09920

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Mouse

    • Sequence

      VPLVTVSALPPSLPLPRSFLLKSLEQVRKIQASGSVLLEQLCATYKLCHP EELVLLGHSLGIPKASLSGCSSQALQQTQCLSQLHSGLCLYQGLLQALSG ISPALAPTLDLLQLDVANFATTIWQQMENLGVAPTVQPTQSAMPAFTSAF QRRAGGVLAISYLQGFLETARLALHHLA

    • Predicted molecular weight

      19 kDa

    • Amino acids

      31 to 208

    • Additional sequence information

      This product is the mature full length protein from aa 31 to 208. The signal peptide is not included.

Specifications

Our Abpromise guarantee covers the use of ab243120 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

    HPLC

  • Form

    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 4.00
    Constituents: 0.29% Sodium citrate, 0.87% Sodium chloride, 0.01% Tween

    0.2 m filtered.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Briefly centrifuge prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20 °C or below. Further dilutions should be made in appropriate buffered solutions.

General Info

  • Alternative names

    • C17orf33
    • Colony stimulating factor 3
    • Colony stimulating factor 3 (granulocyte)
    • CSF 3
    • CSF beta
    • CSF3
    • CSF3_HUMAN
    • CSF3OS
    • Csfg
    • Filgrastim
    • G-CSF
    • GCSA
    • GCSF
    • Granulocyte colony stimulating factor
    • Granulocyte colony-stimulating factor
    • Lenograstim
    • Macrophage granulocyte inducer 2
    • MGC45931
    • MGI 2
    • Pluripoietin
    see all

  • Function

    Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.

  • Sequence similarities

    Belongs to the IL-6 superfamily.

  • Post-translational
    modifications

    O-glycan consists of Gal-GalNAc disaccharide which can be modified with up to two sialic acid residues (done in recombinantly expressed G-CSF from CHO cells).

  • Cellular localization

    Secreted.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant mouse G-CSF protein (Active) (ab243120)
    SDS-PAGE - Recombinant mouse G-CSF protein (Active) (ab243120)
    SDS Page analysis of ab243120

References

ab243120 has not yet been referenced specifically in any publications.

Publishing research using ab243120? Please let us know so that we can cite the reference in this datasheet.

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