Bioactive grade

Recombinant mouse G-CSF protein (Active) (ab243120)

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SDS-PAGE - Recombinant mouse G-CSF protein (Active) (ab243120)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 98% SDS-PAGE
    • Endotoxin level: < 1.000 Eu/µg
    • Active: Yes
    • Suitable for: Functional Studies, SDS-PAGE, HPLC

    Description

    • Product name

      Recombinant mouse G-CSF protein (Active)
      See all G-CSF proteins and peptides

    • Biological activity

      Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine NFS 60 cells is less than 0.05 ng/ml, corresponding to a specific activity of >2.0x107 IU/mg.

    • Purity

      > 98 % SDS-PAGE.
      >98 % by HPLC.

    • Endotoxin level

      < 1.000 Eu/µg

    • Expression system

      Escherichia coli

    • Accession

      P09920

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Mouse

      • Sequence

        VPLVTVSALPPSLPLPRSFLLKSLEQVRKIQASGSVLLEQLCATYKLCHP EELVLLGHSLGIPKASLSGCSSQALQQTQCLSQLHSGLCLYQGLLQALSG ISPALAPTLDLLQLDVANFATTIWQQMENLGVAPTVQPTQSAMPAFTSAF QRRAGGVLAISYLQGFLETARLALHHLA

      • Predicted molecular weight

        19 kDa

      • Amino acids

        31 to 208

      • Additional sequence information

        This product is the mature full length protein from aa 31 to 208. The signal peptide is not included.

    Associated products

      Specifications

      Our Abpromise guarantee covers the use of ab243120 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      • Applications

        Functional Studies

        SDS-PAGE

        HPLC

      • Form

        Lyophilized
      • Concentration information loading...

      Preparation and Storage

      • Stability and Storage

        Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

        Constituents: 0.29% Sodium citrate, 0.87% Sodium chloride, 0.01% Tween

        0.2 m filtered.

        This product is an active protein and may elicit a biological response in vivo, handle with caution.

      • Reconstitution
        Briefly centrifuge prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20 °C or below. Further dilutions should be made in appropriate buffered solutions.

      General Info

      • Alternative names

        • C17orf33
        • Colony stimulating factor 3
        • Colony stimulating factor 3 (granulocyte)
        • CSF 3
        • CSF beta
        • CSF3
        • CSF3_HUMAN
        • CSF3OS
        • Csfg
        • Filgrastim
        • G-CSF
        • GCSA
        • GCSF
        • Granulocyte colony stimulating factor
        • Granulocyte colony-stimulating factor
        • Lenograstim
        • Macrophage granulocyte inducer 2
        • MGC45931
        • MGI 2
        • Pluripoietin
        see all

      • Function

        Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.

      • Sequence similarities

        Belongs to the IL-6 superfamily.

      • Post-translational
        modifications

        O-glycan consists of Gal-GalNAc disaccharide which can be modified with up to two sialic acid residues (done in recombinantly expressed G-CSF from CHO cells).

      • Cellular localization

        Secreted.
      • Information by UniProt

      Images

      • SDS-PAGE - Recombinant mouse G-CSF protein (Active) (ab243120)
        SDS-PAGE - Recombinant mouse G-CSF protein (Active) (ab243120)
        SDS Page analysis of ab243120

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References (0)

      Publishing research using ab243120? Please let us know so that we can cite the reference in this datasheet.

      ab243120 has not yet been referenced specifically in any publications.

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