Recombinant Mouse Galectin 3 protein (ab137144)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: MS, SDS-PAGE
Description
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Product name
Recombinant Mouse Galectin 3 protein
See all Galectin 3 proteins and peptides -
Purity
> 95 % SDS-PAGE.
ab137144 is purified using conventional chromatography techniques. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Mouse -
Sequence
MGSSHHHHHH SSGLVPRGSH MGSMADSFSL NDALAGSGNP NPQGYPGAWG NQPGAGGYPG AAYPGAYPGQ APPGAYPGQA PPGAYPGQAP PSAYPGPTAP GAYPGPTAPG AYPGSTAPGA FPGQPGAPGA YPSAPGGYPA AGPYGVPAGP LTVPYDLPLP GGVMPRMLIT IMGTVKPNAN RIVLDFRRGN DVAFHFNPRF NENNRRVIVC NTKQDNNWGK EERQSAFPFE SGKPFKIQVL VEADHFKVAV NDAHLLQYNH RMKNLREISQ LGISGDITLT SANHAMI -
Predicted molecular weight
30 kDa including tags -
Amino acids
1 to 264 -
Tags
His tag N-Terminus
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Associated products
-
Related Products
Specifications
Our Abpromise guarantee covers the use of ab137144 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Mass Spectrometry
SDS-PAGE
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Mass spectrometry
MALDI-TOF -
Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.02% DTT, 0.32% Tris HCl, 0.06% EDTA, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
General Info
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Alternative names
- 35 kDa lectin
- Carbohydrate binding protein 35
- Carbohydrate-binding protein 35
see all -
Function
Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. -
Tissue specificity
A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages. -
Sequence similarities
Contains 1 galectin domain. -
Cellular localization
Nucleus. Cytoplasmic in adenomas and carcinomas. May be secreted by a non-classical secretory pathway and associate with the cell surface. - Information by UniProt
Images
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SDS-PAGE - Recombinant Mouse Galectin 3 protein (ab137144)
15% SDS-PAGE analysis of 3 µg ab137144.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Recombinant Mouse Galectin 3 protein (ab137144)Immunohistochemical analysis of paraffin embedded Normal Human Brain tissue using ab137144 showing -ve staining. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Recombinant Mouse Galectin 3 protein (ab137144)Immunohistochemical analysis of paraffin embedded Human Breast carcinoma tissue using ab137144 showing +ve staining. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Recombinant Mouse Galectin 3 protein (ab137144)Immunohistochemical analysis of paraffin embedded Normal Human Uterus tissue using ab137144 showing +ve staining. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Recombinant Mouse Galectin 3 protein (ab137144)Immunohistochemical analysis of paraffin embedded Normal Human Tonsil tissue using ab137144 showing +ve staining.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab137144 has not yet been referenced specifically in any publications.
