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    recombinant-mouse-galectin-3-protein-ab137144.pdf

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Recombinant Mouse Galectin 3 protein (ab137144)

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SDS-PAGE - Recombinant Mouse Galectin 3 protein (ab137144)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Recombinant Mouse Galectin 3 protein (ab137144)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Recombinant Mouse Galectin 3 protein (ab137144)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Recombinant Mouse Galectin 3 protein (ab137144)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Recombinant Mouse Galectin 3 protein (ab137144)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 95% SDS-PAGE
  • Tags: His tag N-Terminus
  • Suitable for: MS, SDS-PAGE

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Description

  • Product name

    Recombinant Mouse Galectin 3 protein
    See all Galectin 3 proteins and peptides
  • Purity

    > 95 % SDS-PAGE.
    ab137144 is purified using conventional chromatography techniques.
  • Expression system

    Escherichia coli
  • Accession

    P16110
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Mouse
    • Sequence

      MGSSHHHHHH SSGLVPRGSH MGSMADSFSL NDALAGSGNP NPQGYPGAWG NQPGAGGYPG AAYPGAYPGQ APPGAYPGQA PPGAYPGQAP PSAYPGPTAP GAYPGPTAPG AYPGSTAPGA FPGQPGAPGA YPSAPGGYPA AGPYGVPAGP LTVPYDLPLP GGVMPRMLIT IMGTVKPNAN RIVLDFRRGN DVAFHFNPRF NENNRRVIVC NTKQDNNWGK EERQSAFPFE SGKPFKIQVL VEADHFKVAV NDAHLLQYNH RMKNLREISQ LGISGDITLT SANHAMI
    • Predicted molecular weight

      30 kDa including tags
    • Amino acids

      1 to 264
    • Tags

      His tag N-Terminus

Associated products

  • Related Products

    • Anti-6X His tag® antibody [HIS.H8] (ab18184)
    • Anti-6X His tag® antibody [4D11] (ab5000)
    • Anti-6X His tag® antibody (ab9108)
    • Biotin Anti-Galectin 3 antibody [M3/38] (ab95679)
    • PE Anti-Galectin 3 antibody [M3/38] (ab95680)

Specifications

Our Abpromise guarantee covers the use of ab137144 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Mass Spectrometry

    SDS-PAGE

  • Mass spectrometry

    MALDI-TOF
  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.02% DTT, 0.32% Tris HCl, 0.06% EDTA, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

General Info

  • Alternative names

    • 35 kDa lectin
    • Carbohydrate binding protein 35
    • Carbohydrate-binding protein 35
    • CBP 35
    • CBP35
    • Gal-3
    • GAL3
    • Galactose-specific lectin 3
    • Galactoside-binding protein
    • GALBP
    • Galectin 3 internal gene,included
    • Galectin-3
    • Galectin3
    • GALIG
    • GBP
    • IgE binding protein
    • IgE-binding protein
    • L 31
    • L 34
    • L-31
    • L-34 galactoside-binding lectin
    • L31
    • Laminin-binding protein
    • Lectin L-29
    • Lectin, galactose binding, soluble 3
    • LEG3_HUMAN
    • LGALS2
    • LGALS3
    • MAC 2 antigen
    • Mac-2
    • Mac-2 antigen
    • MAC2
    • Macrophage galactose-specific lectin
    • MGC105387
    see all
  • Function

    Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis.
  • Tissue specificity

    A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages.
  • Sequence similarities

    Contains 1 galectin domain.
  • Cellular localization

    Nucleus. Cytoplasmic in adenomas and carcinomas. May be secreted by a non-classical secretory pathway and associate with the cell surface.
  • Target information above from: UniProt accession P17931 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

Images

  • SDS-PAGE - Recombinant Mouse Galectin 3 protein (ab137144)
    SDS-PAGE - Recombinant Mouse Galectin 3 protein (ab137144)

    15% SDS-PAGE analysis of 3 µg ab137144.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Recombinant Mouse Galectin 3 protein (ab137144)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Recombinant Mouse Galectin 3 protein (ab137144)
    Immunohistochemical analysis of paraffin embedded Normal Human Brain tissue using ab137144 showing -ve staining.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Recombinant Mouse Galectin 3 protein (ab137144)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Recombinant Mouse Galectin 3 protein (ab137144)
    Immunohistochemical analysis of paraffin embedded Human Breast carcinoma tissue using ab137144 showing +ve staining.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Recombinant Mouse Galectin 3 protein (ab137144)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Recombinant Mouse Galectin 3 protein (ab137144)
    Immunohistochemical analysis of paraffin embedded Normal Human Uterus tissue using ab137144 showing +ve staining.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Recombinant Mouse Galectin 3 protein (ab137144)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Recombinant Mouse Galectin 3 protein (ab137144)
    Immunohistochemical analysis of paraffin embedded Normal Human Tonsil tissue using ab137144 showing +ve staining.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab137144? Please let us know so that we can cite the reference in this datasheet.

    ab137144 has not yet been referenced specifically in any publications.

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