For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    recombinant-mouse-gapdh-protein-ab202148.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Neuroscience Neurology process Neurodegenerative disease Huntington's disease
Share by email

Recombinant Mouse GAPDH protein (ab202148)

  • Datasheet
  • SDS
Submit a review Submit a question

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

SDS-PAGE - Recombinant Mouse GAPDH protein (ab202148)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 95% SDS-PAGE
    • Tags: His tag N-Terminus
    • Suitable for: SDS-PAGE, MS

    You may also be interested in

    Primary
    Product image
    Anti-SLC34A3 antibody [EPR9588] (ab155986)
    Assay
    Product image
    Glyceraldehyde 3 Phosphate Dehydrogenase Activity Assay Kit (Colorimetric) (ab204732)
    Protein
    Product image
    Recombinant human PKM2 protein (Active) (ab89364)

    View more associated products

    Description

    • Product name

      Recombinant Mouse GAPDH protein
      See all GAPDH proteins and peptides
    • Purity

      > 95 % SDS-PAGE.
      ab202148 was purified using conventional chromatography techniques.
    • Expression system

      Escherichia coli
    • Accession

      P16858
    • Protein length

      Full length protein
    • Animal free

      No
    • Nature

      Recombinant
      • Species

        Mouse
      • Sequence

        MGSSHHHHHHSSGLVPRGSHMGSMVKVGVNGFGRIGRLVTRAAICSGKVE IVAINDPFIDLNYMVYMFQYDSTHGKFNGTVKAENGKLVINGKPITIFQE RDPTNIKWGEAGAEYVVESTGVFTTMEKAGAHLKGGAKRVIISAPSADAP MFVMGVNHEKYDNSLKIVSNASCTTNCLAPLAKVIHDNFGIVEGLMTTVH AITATQKTVDGPSGKLWRDGRGAAQNIIPASTGAAKAVGKVIPELNGKLT GMAFRVPTPNVSVVDLTCRLEKPAKYDDIKKVVKQASEGPLKGILGYTED QVVSCDFNSNSHSSTFDAGAGIALNDNFVKLISWYDNEYGYSNRVVDLMA YMASKE
      • Predicted molecular weight

        38 kDa including tags
      • Amino acids

        1 to 333
      • Tags

        His tag N-Terminus
      • Additional sequence information

        NP_032110.

    Associated products

    • Related Products

      • Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)
      • Anti-GAPDH antibody [EPR16884] - Loading Control (ab181603)
      • Anti-6X His tag® antibody [HIS.H8] (ab18184)
      • Anti-6X His tag® antibody [4D11] (ab5000)
      • Anti-GAPDH antibody - Loading Control (ab70700)
      • Anti-6X His tag® antibody (ab9108)

    Specifications

    Our Abpromise guarantee covers the use of ab202148 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      Mass Spectrometry

    • Mass spectrometry

      MALDI-TOF
    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 7.40
      Constituents: 20% Glycerol (glycerin, glycerine), 0.02% DTT, 79% PBS

    General Info

    • Alternative names

      • 38 kDa BFA-dependent ADP-ribosylation substrate
      • aging associated gene 9 protein
      • Aging-associated gene 9 protein
      • BARS-38
      • cb609
      • EC 1.2.1.12
      • Epididymis secretory sperm binding protein Li 162eP
      • G3P_HUMAN
      • G3PD
      • G3PDH
      • GAPD
      • GAPDH
      • Glyceraldehyde 3 phosphate dehydrogenase
      • glyceraldehyde 3-PDH
      • Glyceraldehyde-3-phosphate dehydrogenase
      • HEL-S-162eP
      • KNC-NDS6
      • MGC102544
      • MGC102546
      • MGC103190
      • MGC103191
      • MGC105239
      • MGC127711
      • MGC88685
      • OCAS, p38 component
      • OCT1 coactivator in S phase, 38-KD component
      • peptidyl cysteine S nitrosylase GAPDH
      • Peptidyl-cysteine S-nitrosylase GAPDH
      • wu:fb33a10
      see all
    • Function

      Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
    • Pathway

      Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
    • Sequence similarities

      Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
    • Post-translational
      modifications

      S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
      ISGylated.
    • Cellular localization

      Cytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.
    • Target information above from: UniProt accession P04406 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    Images

    • SDS-PAGE - Recombinant Mouse GAPDH protein (ab202148)
      SDS-PAGE - Recombinant Mouse GAPDH protein (ab202148)

      15% SDS-PAGE analysis of ab202148 (3 µg).

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab202148? Please let us know so that we can cite the reference in this datasheet.

    ab202148 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab202148.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2021 Abcam plc. All rights reserved.