Recombinant Mouse GAPDH protein (ab202148)
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Overview
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Product nameRecombinant Mouse GAPDH protein
See all GAPDH proteins and peptides -
Protein lengthFull length protein
Description
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NatureRecombinant
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SourceEscherichia coli
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Amino Acid Sequence
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Accession
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SpeciesMouse
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SequenceMGSSHHHHHHSSGLVPRGSHMGSMVKVGVNGFGRIGRLVTRAAICSGKVE IVAINDPFIDLNYMVYMFQYDSTHGKFNGTVKAENGKLVINGKPITIFQE RDPTNIKWGEAGAEYVVESTGVFTTMEKAGAHLKGGAKRVIISAPSADAP MFVMGVNHEKYDNSLKIVSNASCTTNCLAPLAKVIHDNFGIVEGLMTTVH AITATQKTVDGPSGKLWRDGRGAAQNIIPASTGAAKAVGKVIPELNGKLT GMAFRVPTPNVSVVDLTCRLEKPAKYDDIKKVVKQASEGPLKGILGYTED QVVSCDFNSNSHSSTFDAGAGIALNDNFVKLISWYDNEYGYSNRVVDLMA YMASKE
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Molecular weight38 kDa including tags
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Amino acids1 to 333
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TagsHis tag N-Terminus
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Additional sequence informationNP_032110.
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Associated products
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Related Products
- GAPDH ELISA Kit (ab176642)
- Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)
- Anti-GAPDH antibody [EPR16884] - Loading Control (ab181603)
- Anti-6X His tag® antibody [HIS.H8] (ab18184)
- Anti-6X His tag® antibody [4D11] (ab5000)
- Anti-GAPDH antibody - Loading Control (ab70700)
- Anti-6X His tag® antibody - ChIP Grade (ab9108)
Specifications
Our Abpromise guarantee covers the use of ab202148 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Mass Spectrometry
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Mass spectrometryMALDI-TOF
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Purity>95% by SDS-PAGE .
ab202148 was purified using conventional chromatography techniques. -
FormLiquid
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: 20% Glycerol, 0.02% DTT, 79% PBS
General Info
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Alternative names
- 38 kDa BFA-dependent ADP-ribosylation substrate
- aging associated gene 9 protein
- Aging-associated gene 9 protein
see all -
FunctionHas both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
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PathwayCarbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
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Sequence similaritiesBelongs to the glyceraldehyde-3-phosphate dehydrogenase family.
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Post-translational
modificationsS-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
ISGylated. -
Cellular localizationCytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.
- Information by UniProt
Images
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SDS-PAGE - Recombinant Mouse GAPDH protein (ab202148)15% SDS-PAGE analysis of ab202148 (3 µg).
Datasheets and documents
References
ab202148 has not yet been referenced specifically in any publications.
