Overview

Description

  • Nature

    Recombinant
  • Source

    Yeast
  • Amino Acid Sequence
    • Accession
    • Species

      Mouse
    • Sequence

      APTRSPITVT RPWKHVEAIK EALNLLDDMP VTLNEEVEVV SNEFSFKKLT CVQTRLKIFE QGLRGNFTKL KGALNMTASY YQTYCPPTPE TDCETQVTTY ADFIDSLKTF LTDIPFECKK PGQK
    • Molecular weight

      14 kDa
    • Amino acids

      18 to 141

Specifications

Our Abpromise guarantee covers the use of ab136380 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Purity

    > 95 % SDS-PAGE.
    ab136380 was purified by Ion-exchange chromatography.
  • Form

    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C. Avoid freeze / thaw cycle.

    Constituents: 89% PBS, 10% Trehalose

  • Reconstitution
    Reconstitute with sterile phosphate-buffered saline containing at least 0.1% carrier protein.

General Info

  • Alternative names

    • Colony stimulating factor
    • Colony Stimulating Factor 2
    • Colony stimulating factor 2 (granulocyte-macrophage)
    • Colony-stimulating factor
    • CSF
    • CSF 2
    • CSF2
    • CSF2_HUMAN
    • GM-CSF
    • GMCSF
    • Granulocyte Macrophage Colony Stimulating Factor
    • Granulocyte-macrophage colony-stimulating factor
    • MGC131935
    • MGC138897
    • MGI1GM
    • Molgramostin
    • Pluripoietin-a
    • Sargramostim
    see all
  • Function

    Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
  • Sequence similarities

    Belongs to the GM-CSF family.
  • Cellular localization

    Secreted.
  • Information by UniProt

References

ab136380 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer


This protein is generated in yeast. Yeast proteins have the potential to be post-translationally modified. This modification will lead to various forms of the protein at different molecular weights.

The competitor proteins are most likely generated by a non-mammalian system, such as E. coli. E. coli does not post-translationally modify their proteins, so there would be no modification with E. coli proteins.

Typically, more post-translational modifications corresponds to more biological activity of a protein (a desired response).

In specific response to this particular protein, Mouse GM-CSF, all of our mammalian GM-CSF proteins (mouse, equine, and bovine) demonstrate a similar multi-band profile in the final SDS-gel.

Read More

Answer

Thank you for contacting us.

The mouse GM-CSF recombinant protein that we produce does not contain a methionine at the amino terminus. We are producing the mature form which starts with alanine at position 18.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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