Recombinant mouse Granzyme B protein (ab50114)

Product name

Recombinant mouse Granzyme B protein
See all Granzyme B proteins and peptides
Protein length

Protein fragment

Our Abpromise guarantee covers the use of ab50114 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Biological activity

Determined by its ability to cleave a synthetic chromogentic Granzyme B substrate. The expected specific activity, when using the Ac-IEPD-pNA substrate at 25°C, is greater than 750 nM/min per µg of enzyme.
Applications

SDS-PAGE

Functional Studies
Endotoxin level

< 0.100 Eu/µg
Purity

> 95 % SDS-PAGE.
Greater than 98% by SDS-PAGE and HPLC analyses.
Form

Lyophilised
Concentration information loading...

Stability and Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Preservative: None
Endotoxin level is less than 0.1 ng per µg (1EU/µg).

This product is an active protein and may elicit a biological response in vivo, handle with caution.
Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4oC for 1 week or -20oC for future use.

Alternative names
- C11
- Cathepsin G like 1
- Cathepsin G-like 1
- CCPI
- CGL 1
- CGL1
- CSP B
- CSPB
- CTLA-1
- CTLA1
- CTSGL1
- Cytotoxic serine protease B
- Cytotoxic T lymphocyte associated serine esterase 1
- Cytotoxic T lymphocyte proteinase 2
- Cytotoxic T-lymphocyte proteinase 2
- Fragmentin 2
- Fragmentin-2
- GRAB_HUMAN
- Granzyme 2
- Granzyme B
- Granzyme B (granzyme 2, cytotoxic T lymphocyte associated serine esterase 1)
- Granzyme-2
- GranzymeB
- GRB
- Gzmb
- Hlp
- Human lymphocyte protein
- Lymphocyte protease
- Protease, serine, B
- SECT
- T cell serine protease 1-3E
- T-cell serine protease 1-3E
see all
Function

This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.
Sequence similarities

Belongs to the peptidase S1 family. Granzyme subfamily.
Contains 1 peptidase S1 domain.
Cellular localization

Cytoplasmic granule. Cytoplasmic granules of cytolytic T-lymphocytes and natural killer cells.
Target information above from: UniProt accession P10144 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt

ab50114 has not yet been referenced specifically in any publications.

Publishing research using ab50114? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As