Recombinant mouse Granzyme B protein (ab50114)

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Description

  • Product name

    Recombinant mouse Granzyme B protein
    See all Granzyme B proteins and peptides

  • Biological activity

    Determined by its ability to cleave a synthetic chromogentic Granzyme B substrate. The expected specific activity, when using the Ac-IEPD-pNA substrate at 25°C, is greater than 750 nM/min per µg of enzyme.

  • Purity

    > 95 % SDS-PAGE.
    Greater than 98% by SDS-PAGE and HPLC analyses.

  • Endotoxin level

    < 0.100 Eu/µg

  • Expression system

    Baculovirus infected BTI-TN-5B1-4 cells

  • Accession

    P04187

  • Protein length

    Protein fragment

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Mouse

    • Sequence

      IIGGHEVKPH SRPYMALLSI KDQQPEAICG GFLIREDFVL TAAHCEGSII NVTLGAHNIK EQEKTQQVIP MVKCIPHPDY NPKTFSNDIM LLKLKSKAKR TRAVRPLNLP RRNVNVKPGD VCYVAGWGRM APMGKYSNTL QEVELTVQKD RECESYFKNR YNKTNQICAG DPKTKRASFR GDSGGPLVCK KVAAGIVSYG YKDGSPPRAF TKVSSFLSWI KKTMKSS

Associated products

    Specifications

    Our Abpromise guarantee covers the use of ab50114 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      Functional Studies

    • Form

      Lyophilized
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

      Preservative: None
      Endotoxin level is less than 0.1 ng per µg (1EU/µg).

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    • Reconstitution
      Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4oC for 1 week or -20oC for future use.

    General Info

    • Alternative names

      • C11
      • Cathepsin G like 1
      • Cathepsin G-like 1
      • CCPI
      • CGL 1
      • CGL1
      • CSP B
      • CSPB
      • CTLA 1
      • CTLA-1
      • CTLA1
      • CTSGL1
      • Cytotoxic serine protease B
      • Cytotoxic T lymphocyte associated serine esterase 1
      • Cytotoxic T lymphocyte proteinase 2
      • Cytotoxic T-lymphocyte proteinase 2
      • Fragmentin 2
      • Fragmentin-2
      • GRAB_HUMAN
      • Granzyme 2
      • Granzyme B
      • Granzyme B (granzyme 2, cytotoxic T lymphocyte associated serine esterase 1)
      • Granzyme-2
      • GranzymeB
      • GRB
      • Gzmb
      • Hlp
      • Human lymphocyte protein
      • Lymphocyte protease
      • Protease, serine, B
      • SECT
      • T cell serine protease 1 3E
      • T cell serine protease 1-3E
      • T-cell serine protease 1-3E
      see all

    • Function

      This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.

    • Sequence similarities

      Belongs to the peptidase S1 family. Granzyme subfamily.
      Contains 1 peptidase S1 domain.

    • Cellular localization

      Cytoplasmic granule. Cytoplasmic granules of cytolytic T-lymphocytes and natural killer cells.
    • Information by UniProt

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab50114? Please let us know so that we can cite the reference in this datasheet.

    ab50114 has not yet been referenced specifically in any publications.

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