Recombinant mouse Granzyme B protein (ab50114)

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Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected BTI-TN-5B1-4 cells
  • Amino Acid Sequence
    • Species
      Mouse

Specifications

Our Abpromise guarantee covers the use of ab50114 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Determined by its ability to cleave a synthetic chromogentic Granzyme B substrate. The expected specific activity, when using the Ac-IEPD-pNA substrate at 25°C, is greater than 750 nM/min per µg of enzyme.
  • Applications

    SDS-PAGE

    Functional Studies

  • Endotoxin level
    < 0.100 Eu/µg
  • Purity
    > 95 % SDS-PAGE.
    Greater than 98% by SDS-PAGE and HPLC analyses.
  • Form
    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Endotoxin level is less than 0.1 ng per µg (1EU/µg).

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4oC for 1 week or -20oC for future use.

General Info

  • Alternative names
    • C11
    • Cathepsin G like 1
    • Cathepsin G-like 1
    • CCPI
    • CGL 1
    • CGL1
    • CSP B
    • CSPB
    • CTLA-1
    • CTLA1
    • CTSGL1
    • Cytotoxic serine protease B
    • Cytotoxic T lymphocyte associated serine esterase 1
    • Cytotoxic T lymphocyte proteinase 2
    • Cytotoxic T-lymphocyte proteinase 2
    • Fragmentin 2
    • Fragmentin-2
    • GRAB_HUMAN
    • Granzyme 2
    • Granzyme B
    • Granzyme B (granzyme 2, cytotoxic T lymphocyte associated serine esterase 1)
    • Granzyme-2
    • GranzymeB
    • GRB
    • Gzmb
    • Hlp
    • Human lymphocyte protein
    • Lymphocyte protease
    • Protease, serine, B
    • SECT
    • T cell serine protease 1-3E
    • T-cell serine protease 1-3E
    see all
  • Function
    This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.
  • Sequence similarities
    Belongs to the peptidase S1 family. Granzyme subfamily.
    Contains 1 peptidase S1 domain.
  • Cellular localization
    Cytoplasmic granule. Cytoplasmic granules of cytolytic T-lymphocytes and natural killer cells.
  • Information by UniProt

References

ab50114 has not yet been referenced specifically in any publications.

Publishing research using ab50114? Please let us know so that we can cite the reference in this datasheet.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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