Description

  • Product name

    Recombinant mouse Hsp60 protein
    See all Hsp60 proteins and peptides
  • Biological activity

    ATPase Activity Assay: Positive
  • Purity

    > 90 % SDS-PAGE.
    >90% pure as determined by SDS-PAGE and Western blot analyses. This protein does not contain E. coli GroEL as demonstrated by western blot analysis.
  • Expression system

    Escherichia coli
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Mouse

Specifications

Our Abpromise guarantee covers the use of ab92364 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    Functional Studies

    SDS-PAGE

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.0154% DTT, 0.158% Tris HCl, 0.0292% EDTA, 0.87% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • 60 kDa chaperonin
    • 60 kDa heat shock protein, mitochondrial
    • CH60_HUMAN
    • Chaperonin 60
    • Chaperonin, 60-KD
    • CPN60
    • fa04a05
    • GROEL
    • heat shock 60kDa protein 1 (chaperonin)
    • Heat shock protein 1 (chaperonin)
    • Heat shock protein 60
    • Heat shock protein 65
    • heat shock protein family D (Hsp60) member 1
    • HLD4
    • Hsp 60
    • HSP 65
    • HSP-60
    • HSP60
    • HSP65
    • HSPD1
    • HuCHA60
    • Mitochondrial matrix protein P1
    • P60 lymphocyte protein
    • short heat shock protein 60 Hsp60s1
    • SPG13
    see all
  • Function

    Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.
  • Involvement in disease

    Defects in HSPD1 are a cause of spastic paraplegia autosomal dominant type 13 (SPG13) [MIM:605280]. Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs.
    Defects in HSPD1 are the cause of leukodystrophy hypomyelinating type 4 (HLD4) [MIM:612233]; also called mitochondrial HSP60 chaperonopathy or MitCHAP-60 disease. HLD4 is a severe autosomal recessive hypomyelinating leukodystrophy. Clinically characterized by infantile-onset rotary nystagmus, progressive spastic paraplegia, neurologic regression, motor impairment, profound mental retardation. Death usually occurrs within the first two decades of life.
  • Sequence similarities

    Belongs to the chaperonin (HSP60) family.
  • Cellular localization

    Mitochondrion matrix.
  • Information by UniProt

Images

  • SDS-PAGE Analysis:
    Lane 1: Molecular weight markers
    Lane 2: ab92364 at 2.0 μg
  • All lanes : Hsp60 monoclonal antibody at 1 µg/ml

    Lane 1 : Molecular weight markers
    Lane 2 : ab92364 at 0.1 µg
    Lane 3 : E. coli GroEL Protein at 0.1 µg
  • All lanes : GroEL monoclonal antibody at 1 µg/ml

    Lane 1 : Molecular weight markers
    Lane 2 : ab92364 at 0.1 µg
    Lane 3 : E. coli GroEL Protein at 0.1 µg

References

ab92364 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for yor enquiry and interest in our products.

Currently, we have a few purified Hsp60 proteins (9) in our catalogue such as ab113192, ab113177, ab92365.

You may need to consider the species of the protein (human or mouse or rat) to make sure that the primary antibody does recognize it. You have only mentioned the host species of the primary which is mouse but please do check which species Anti-hsp60 primary antibody detects.

If you need any further assistance in the future, please do not hesitate to contact me.

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