Overview

Description

  • Nature

    Recombinant
  • Source

    CHO cells
  • Amino Acid Sequence
    • Accession
    • Species

      Mouse
    • Sequence

      TEVGAMVGSNVVLSCIDPHRRHFNLSGLYVYWQIENPEVSVTYYLPYKSP GINVDSSYKNRGHLSLDSMKQGNFSLYLKNVTPQDTQEFTCRVFMNTATE LVKILEEVVRLRVAANFSTPVISTSDSSNPGQERTYTCMSKNGYPEPNLY WINTTDNSLIDTALQNNTVYLNKLGLYDVISTLRLPWTSRGDVLCCVENV ALHQNITSISQAESFTGNNTKNPQETHNNELK
    • Amino acids

      48 to 279
    • Additional sequence information

      Extracellular domain fused to the N-terminus of the Fc region of a mutant mouse IgG2a.

Specifications

Our Abpromise guarantee covers the use of ab214990 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    Measured by its binding ability in a functional ELISA. Immobilized human ICOS at 1μg/ml (100μl/well) can bind biotinylated B7-H2 (mouse):Fc (mouse) (rec.) (non-lytic) with a linear range of 0.1-1.0μg/ml. Optimal dilutions should be determined by each laboratory for each application.

    Acts as a long lasting fusion protein which only binds to the receptor. Mutations to the complement (C1q) and FcγR I binding sites of the IgGs Fc fragment render the fusion proteins incapable of antibody directed cytotoxicity (ADCC) and complement directed cytotoxicity (CDC).

  • Applications

    SDS-PAGE

    Functional Studies

  • Endotoxin level

    < 0.060 Eu/µg
  • Purity

    >= 98 % SDS-PAGE.

  • Form

    Lyophilised
  • Additional notes

     This product was previously labelled as ICOS Ligand

     

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C.

    Constituent: 100% PBS

    Lyophilized from 0.2µm-filtered solution

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Reconstitute at 100µg/ml in sterile PBS.

General Info

  • Alternative names

    • B7 H2
    • B7 homolog 2
    • B7 homologue 2
    • B7 like protein Gl50
    • B7 related protein 1
    • B7-H2
    • B7-like protein Gl50
    • B7-related protein 1
    • B7H2
    • B7RP 1
    • B7RP-1
    • B7RP1
    • CD 275
    • CD275
    • CD275 antigen
    • GL 50
    • GL50
    • ICOS L
    • ICOS LG
    • ICOS ligand
    • ICOSL
    • ICOSL_HUMAN
    • Icoslg
    • Inducible T cell co stimulator ligand
    • KIAA0653
    • LICOS
    • Transmembrane protein B7 H2 ICOS ligand
    see all
  • Function

    Ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells. Could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function.
  • Tissue specificity

    Isoform 1 is widely expressed (brain, heart, kidney, liver, lung, pancreas, placenta, skeletal muscle, bone marrow, colon, ovary, prostate, testis, lymph nodes, leukocytes, spleen, thymus and tonsil), while isoform 2 is detected only in lymph nodes, leukocytes and spleen. Expressed on activated monocytes and dendritic cells.
  • Sequence similarities

    Belongs to the immunoglobulin superfamily. BTN/MOG family.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization

    Membrane.
  • Information by UniProt

References

ab214990 has not yet been referenced specifically in any publications.

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