Recombinant mouse IL-10 protein (Active) (ab281800)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 95% SDS-PAGE
- Endotoxin level: <= 0.005 Eu/µg
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE, MS, HPLC
Description
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Product name
Recombinant mouse IL-10 protein (Active)
See all IL-10 proteins and peptides -
Biological activity
Fully biologically active determined by dose dependent increase in proliferation of MC/9 cells.
ED50 for this effect is ≤ 0.330 ng/ml corresponding to a specific activity of 3.03 X 106 units/mg.
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Purity
>= 95 % SDS-PAGE.
>= 95% by HPLC -
Endotoxin level
<=0.005 Eu/µg -
Expression system
HEK 293 cells -
Accession
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Protein length
Full length protein -
Animal free
Yes -
Carrier free
Yes -
Nature
Recombinant -
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Species
Mouse -
Sequence
SRGQYSREDN NCTHFPVGQS HMLLELRTAF SQVKTFFQTK DQLDNILLTD SLMQDFKGYL GCQALSEMIQ FYLVEVMPQA EKHGPEIKEH LNSLGEKLKT LRMRLRRCHR FLPCENKSKA VEQVKSDFNK LQDQGVYKAM NEFDIFINCI EAYMMIKMKS -
Predicted molecular weight
19 kDa -
Actual molecular weight
19 kDa -
Molecular weight information
Predicted MW is 18810.75 Da (+/- 10 Da by ESI-TOF). Observed MW is 18812.07 Da. -
Amino acids
19 to 178 -
Additional sequence information
N-terminal glycine Full-length mature chain lacking the signal peptide
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Specifications
Our Abpromise guarantee covers the use of ab281800 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
Mass Spectrometry
HPLC
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at Room Temperature. Store at Room Temperature.
pH: 7.40
Constituents: 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Monobasic dihydrogen potassium phosphate, 10.26% Trehalose
Buffer lyophilized from.This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with phosphate buffered saline.Store lyophilized form at room temperature. Reconstitute, aliquot and store at -80°C for 12 months or +4°C for 1 week.Avoid repeated freeze-thaw. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.
General Info
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Alternative names
- CSIF
- Cytokine synthesis inhibitory factor
- GVHDS
see all -
Function
Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. -
Tissue specificity
Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types. -
Sequence similarities
Belongs to the IL-10 family. -
Cellular localization
Secreted. - Information by UniProt
Images
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Functional Studies - Recombinant mouse IL-10 protein (Active) (ab281800)
Functional analysis of ab281800.
Fully biologically active determined by dose dependent increase in proliferation of MC/9 cells.
ED50 for this effect is ≤ 0.330 ng/ml corresponding to a specific activity of 3.03 X 106 units/mg.
Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.
Lot: GR3387982-1
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SDS-PAGE - Recombinant Mouse IL-10 protein (Tagged) (ab281800)SDS-PAGE analysis of ab281800.
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Mass Spectrometry - Recombinant Mouse IL-10 protein (Tagged) (ab281800)Mass determination by ESI-TOF.
Predicted MW is 18810.75 Da. (+/- 10 Da by ESI-TOF). Observed MW is 18812.07 Da.
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HPLC - Recombinant Mouse IL-10 protein (Tagged) (ab281800)HPLC analysis of ab281800.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab281800 has not yet been referenced specifically in any publications.
