Key features and details
- Expression system: Escherichia coli
- Purity: > 97% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
- High batch-to-batch consistency
- Optimal bioactivity
- Guaranteed identical to human native proteins
- >95% purity
- Ultra-low endotoxin levels: <0.005 Eu/µg
- Carrier and tag free
Product nameRecombinant mouse IL-2 protein
See all IL-2 proteins and peptides
Purity> 97 % SDS-PAGE.
Sterile filtered Greater than 97% pure by HPLC analyses. Endotoxin level is less than 0.1 ng per g (1EU/g).
Expression systemEscherichia coli
Protein lengthFull length protein
SequenceRecombinant Murine IL-2 is a 17.2 kDa protein containing 149 amino acid residues: PTSSSTSSST AEAQQQQQQQ QQQQQHLEQL LMDL QELLSR MENYRNLKLP RMLTFKFYLPKQATELKDLQ CLEDELGPLR HVLDLTQSKS FQLEDAENFI SNIRVTVVKL KGSDNTFECQFDDESAT VVD FLRRWIAFCQ SIISTSPQ
Our Abpromise guarantee covers the use of ab9856 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Additional notesThe ED50, determined by the dose-dependent stimulation of murine CTLL-2 cells, is < 0.2 ng/ml, corresponding to a specific activity of > 5 x 106 units/mg.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionPlease reconstitute this product in 200ul sterile water.
- IL 2
FunctionProduced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
Involvement in diseaseNote=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17.
Sequence similaritiesBelongs to the IL-2 family.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab9856 has been referenced in 1 publication.
- Burr ML et al. An Evolutionarily Conserved Function of Polycomb Silences the MHC Class I Antigen Presentation Pathway and Enables Immune Evasion in Cancer. Cancer Cell 36:385-401.e8 (2019). PubMed: 31564637