Key features and details
- Expression system: Insect cells
- Purity: = 98% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
- High batch-to-batch consistency
- Optimal bioactivity
- Guaranteed identical to human native proteins
- >95% purity
- Ultra-low endotoxin levels: <0.005 Eu/µg
- Carrier and tag free
Product nameRecombinant mouse IL-2 protein (Fc Chimera)
See all IL-2 proteins and peptides
ED50 < 5 ng/ml as determined in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells (Gearing and Bird (1987) Production and assay of IL2. In Lymphokines and Interferons: A Practical Approach (Clemens et al. eds.) pp. 291-301). The ED50 for this effect is typically < 1.0 ng/ml.
Purity= 98 % SDS-PAGE.
ab183262 is purified by chromatography, then sterile filtered.
Expression systemInsect cells
Protein lengthFull length protein
SequenceAPTSSSTSSSTAEAQQQQQQQQQQQQHLEQLLMDLQELLSRMENYRNLKL PRMLTFKFYLPKQATELKDLQCLEDELGPLRHVLDLTQSKSFQLEDAENF ISNIRVTVVKLKGSDNTFECQFDDESATVVDFLRRWIAFCQSIISTSPQ
Predicted molecular weight43 kDa
Amino acids21 to 169
Additional sequence informationMouse IL2 is glycosylated and expressed as a chimera with the Fc region of mouse immunoglobulin (heavy chain hinge, CH2 and CH3).
Our Abpromise guarantee covers the use of ab183262 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Extinction coefficient: 0.9
Produced using non-baculovirus insect cells.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -80°C.
Constituents: 99% PBS, 0.1% BSA
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionAdd 0.025 ml distilled water.
- IL 2
FunctionProduced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
Involvement in diseaseNote=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17.
Sequence similaritiesBelongs to the IL-2 family.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab183262 has not yet been referenced specifically in any publications.