Overview

Description

  • Nature

    Recombinant
  • Source

    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species

      Mouse
    • Sequence

      HKSSPQGPDR LLIRLRHLID IVEQLKIYEN DLDPELLSAP QDVKGHCEHA AFACFQKAKL KPSNPGNNKT FIIDLVAQLR RRLPARRGGK KQKHIAKCPS CDSYEKRTPK EFLERLKWLL QKMIHQHLS
    • Molecular weight

      15 kDa
    • Amino acids

      18 to 146
    • Additional sequence information

      This product is for the mature full length protein from aa 18 to 146. The signal peptide is not included.

Associated products

Specifications

Our Abpromise guarantee covers the use of ab200258 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    The ED50 determined by a cell proliferation assay using human N1186 T cells is less than 25 ng/ml, corresponding to a specific activity of > 4.0 × 10IU/mg.

  • Applications

    SDS-PAGE

    Functional Studies

    HPLC

  • Endotoxin level

    < 1.000 Eu/µg
  • Purity

    > 98 % SDS-PAGE.
    The purity of ab200258 was determined by SDS-PAGE and HPLC analyses.
  • Form

    Lyophilised
  • Additional notes

    This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Further dilutions should be made in appropriate buffered solutions.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C long term. Avoid freeze / thaw cycle. Please see notes section.

    pH: 7.40
    Constituent: 100% PBS

    Lyophilized from a 0.2 um filtered concentrated solution.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml.

General Info

  • Alternative names

    • CVID11
    • IL 21
    • IL-21
    • Il21
    • IL21_HUMAN
    • Interleukin 21
    • Interleukin-21
    • interleukin-21 isoform
    • Interleukin21
    • OTTHUMP00000164088
    • Za11
    see all
  • Function

    Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG(1) and IgG(3) in B-cells (By similarity). May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells. During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation.
  • Tissue specificity

    Expressed in activated CD4-positive T-cells but not in CD8-positive T-cells, B-cells, or monocytes.
  • Sequence similarities

    Belongs to the IL-15/IL-21 family.
  • Cellular localization

    Secreted.
  • Information by UniProt

References

ab200258 has not yet been referenced specifically in any publications.

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