Description

  • Product name

    Recombinant mouse IL-22 protein
    See all IL-22 proteins and peptides
  • Biological activity

    Determined by its ability to induce STAT3 activation in COLO 205 cells. The expected ED50 for this effect is 150-400 pg/ml.

  • Purity

    > 98 % SDS-PAGE.
    Purity is greater than 98% by SDS-PAGE gel and HPLC analyses.
  • Expression system

    Escherichia coli
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Mouse
    • Sequence

      MLPVNTRCKL EVSNFQQPYI VNRTFMLAKE ASLADNNTDV RLIGEKLFRG VSAKDQCYLM KQVLNFTLED VLLPQSDRFQ PYMQEVVPFL TKLSNQLSSC HISGDDQNIQ KNVRRLKETV KKLGESGEIK AIGELDLLFM SLRNACV

Specifications

Our Abpromise guarantee covers the use of ab84083 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Form

    Lyophilised
  • Additional notes

    Endotoxin level is less than 0.1 ng per µg (1EU/µg).
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Reconstituted ab84083 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20oC. Avoid repeated freeze/thaw cycles.

General Info

  • Alternative names

    • Cytokine Zcyto18
    • IL 10 related T cell derived inducible factor
    • IL 21
    • IL 22
    • IL D110
    • IL TIF
    • IL-10-related T-cell-derived-inducible factor
    • IL-22
    • IL-TIF
    • IL21
    • Il22
    • IL22_HUMAN
    • ILD110
    • ILTIF
    • Interleukin 10 related T cell derived inducible factor
    • interleukin 21
    • Interleukin 22
    • Interleukin-22
    • MGC79382
    • MGC79384
    • TIFa
    • TIFIL 23
    • TIFIL23
    • UNQ3099/PRO10096
    • zcyto18
    see all
  • Function

    Cytokine that contributes to the inflammatory response in vivo.
  • Sequence similarities

    Belongs to the IL-10 family.
  • Cellular localization

    Secreted.
  • Information by UniProt

References

This product has been referenced in:

  • Stritesky GL  et al. IL-23 promotes maintenance but not commitment to the Th17 lineage. J Immunol 181:5948-55 (2008). Read more (PubMed: 18941183) »
  • Kreymborg K  et al. IL-22 is expressed by Th17 cells in an IL-23-dependent fashion, but not required for the development of autoimmune encephalomyelitis. J Immunol 179:8098-104 (2007). Read more (PubMed: 18056351) »
See all 2 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for contacting us.

Please check the following link http://www.uniprot.org/uniprot/Q96PD4; IL24 is synonym for IL17F as given in uniprot protein database however the uniprot ID and GeneID for both proteins are different. I am sorry I am unsure why the name used as synonym but different IDS. You may need to search this in literature. The following are the useful links;

http://www.uniprot.org/uniprot/Q13007

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=11009

http://www.uniprot.org/uniprot/Q96PD4

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=112744

The IL17F protein we have are

https://www.abcam.com/IL17F-protein-Human-ab78475.html
https://www.abcam.com/IL17F-protein-Active-ab119722.html
https://www.abcam.com/IL17F-protein-ab50157.html (this is homo-dimer)
https://www.abcam.com/IL17F-protein-Active-ab85600.html

The protein ab40044 is not IL17F it is actualy IL17A or IL17; http://www.uniprot.org/uniprot/Q16552

I have asked lab to confirm, why the signal peptide amino acids are included. I will send you the details soon.

These products are developed by our collaborators and they are from different suppliers which is why the price is different.

Finally, we can provide the product at ab82830 at 0.5mg/ml which is the lower limit at which the product can be used with conjugation kits.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for your phone call.

The protein ab82830 is a monomer; it not homodimer. The amino acid sequence can be found at http://www.uniprot.org with protein ID Q16552.

We unfortunately will not be able to supply this protein as FITC conjugated or PerCP conjugated product. I am sorry our lab will not be able to conjugate these on custom basis. I however have requested lab if they can provide this protein in higher concentration so that the conjugation kits can be used.

We will not be able to provide ab49821, IL22 protein as His tagged.

The IL24 protein we have is ab131696.

I will let you know further when I receive a reply form lab.

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