Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 0.050 Eu/µg
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
Product nameRecombinant mouse IL-22 protein (Animal Free)
See all IL-22 proteins and peptides
Biological activity: the activity is determined by the dose-dependant induction of IL10 from COLO-205 cells and is typically less than 0.2 ng/mL. This corresponds to an expected specific activity of 5 x 106 units/mg.
Purity> 95 % SDS-PAGE.
The purity of ab207981 was determined by reducing and non-reducing SDS-PAGE.
Endotoxin level< 0.050 Eu/µg
Expression systemEscherichia coli
Protein lengthFull length protein
SequenceMLPVNTRCKL EVSNFQQPYI VNRTFMLAKE ASLADNNTDV RLIGEKLFRG VSAKDQCYLM KQVLNFTLED VLLPQSDRFQ PYMQEVVPFL TKLSNQLSSC HISGDDQNIQ KNVRRLKETV KKLGESGEIK AIGELDLLFM SLRNACV
Predicted molecular weight17 kDa
Amino acids34 to 179
Additional sequence informationMature form without initial signal peptide, but with an inital Met.
Our Abpromise guarantee covers the use of ab207981 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Constituent: 0.1% Trifluoroacetic acid
Lyophilized from a sterile filtered aqueous solution.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionReconstitute in sterile water at 0.1 mg/mL Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80 ºC and avoid repeat freeze thaws.
- Cytokine Zcyto18
- IL 10 related T cell derived inducible factor
- IL 21
FunctionCytokine that contributes to the inflammatory response in vivo.
Sequence similaritiesBelongs to the IL-10 family.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab207981 has not yet been referenced specifically in any publications.