Key features and details
- Expression system: Escherichia coli
- Purity: > 98% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: HPLC, Functional Studies, SDS-PAGE
Product nameRecombinant mouse IL-3 protein
See all IL-3 proteins and peptides
The ED50 as determined by the dose-dependent stimulation of the proliferation ofmurine M-NFS-60 cells is < 0.05 ng/ml, corresponding to a specific activity of > 2 x107 units/mg.
Purity> 98 % SDS-PAGE.
ab200268 is >98% pure as assessed by SDS-PAGE and HPLC analyses.
Endotoxin level< 1.000 Eu/µg
Expression systemEscherichia coli
Protein lengthProtein fragment
SequenceMDTHRLTRTLNCSSIVKEIIGKLPEPELKTDDEGPSLRNKSFRRVNLSKF VESQGEVDPEDRYVIKSNLQLNCCLPTSANDSALPGVFIRDLDDFRKKLR FYMVHLNDLETVLTSRPPQPASGSVSPNRGTVEC
Predicted molecular weight15 kDa
Amino acids33 to 166
Our Abpromise guarantee covers the use of ab200268 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Please see notes section. Store under desiccating conditions.
Constituent: 100% PBS
Lyophilized from a 0.2 mm filtered solution.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionWe recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL.
- Colony stimulating factor multiple
- Hematopoietic growth factor
- IL 3
FunctionGranulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages.
This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes.
Tissue specificityActivated T-cells, mast cells, natural killer cells.
Sequence similaritiesBelongs to the IL-3 family.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab200268 has not yet been referenced specifically in any publications.