Recombinant mouse IL-3 protein (Animal Free) (ab179619)
Key features and details
- Expression system: Escherichia coli
- Purity: >= 97% SDS-PAGE
- Endotoxin level: <= 1.000 Eu/µg
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies, HPLC
Description
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Product name
Recombinant mouse IL-3 protein (Animal Free)
See all IL-3 proteins and peptides -
Biological activity
The activity is determined by the dose-dependent stimulation of mouse M-NFS-60 cells and is typically less than 0.05 ng/mL.
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Purity
>= 97 % SDS-PAGE.
Protein Content and Purity determined by HPLC, Reducing and Non-reducing SDS-PAGE, UV spectroscopy at 280 nm -
Endotoxin level
<=1.000 Eu/µg -
Expression system
Escherichia coli -
Accession
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Protein length
Protein fragment -
Animal free
Yes -
Nature
Recombinant -
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Species
Mouse -
Sequence
MDTHRLTRTL NCSSIVKEII GKLPEPELKT DDEGPSLRNK SFRRVNLSKF VESQGEVDPE DRYVIKSNLQ KLNCCLPTSA NDSALPGVFI RDLDDFRKKL RFYMVHLNDL ETVLTSRPPQ PASGSVSPNR GTVEC -
Predicted molecular weight
15 kDa -
Amino acids
33 to 166
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Specifications
Our Abpromise guarantee covers the use of ab179619 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
HPLC
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Form
Lyophilized -
Additional notes
This product is produced with no animal-derived raw products, animal free equipment and animal free protocols.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20°C. Avoid freeze / thaw cycle.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionCentrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/mL, which can be further diluted into other aqueous solutions. Reconstituted material should be aliquoted and frozen at -20°C.
General Info
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Alternative names
- Colony stimulating factor multiple
- Hematopoietic growth factor
- IL 3
see all -
Function
Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages.
This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes. -
Tissue specificity
Activated T-cells, mast cells, natural killer cells. -
Sequence similarities
Belongs to the IL-3 family. -
Cellular localization
Secreted. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab179619 has not yet been referenced specifically in any publications.