Recombinant mouse IL-4 protein (Active) (ab259406)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 95% SDS-PAGE
- Endotoxin level: <= 0.005 Eu/µg
- Active: Yes
- Suitable for: SDS-PAGE, HPLC, Functional Studies, MS
Description
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Product name
Recombinant mouse IL-4 protein (Active)
See all IL-4 proteins and peptides -
Biological activity
Fully biologically active determined by dose dependent increase in HT-2 cell proliferation.
ED50 is ≤1.04 ng/ml, corresponding to a specific activity of 0.96 x 106 units/mg.
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Purity
>= 95 % SDS-PAGE.
=95% by HPLC -
Endotoxin level
<=0.005 Eu/µg -
Expression system
HEK 293 cells -
Accession
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Protein length
Full length protein -
Animal free
Yes -
Carrier free
Yes -
Nature
Recombinant -
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Species
Mouse -
Sequence
HIHGCDKNHL REIIGILNEV TGEGTPCTEM DVPNVLTATK NTTESELVCR ASKVLRIFYL KHGKTPCLKK NSSVLMELQR LFRAFRCLDS SISCTMNESK STSLKDFLES LKSIMQMDYS -
Predicted molecular weight
14 kDa -
Actual molecular weight
14 kDa -
Molecular weight information
Predicted mass is 13615.11 Da (+/- 10 Da by ESI TOF). Observed mass is 13616.32 Da (and + 80 Da due to phosphorylation) -
Amino acids
21 to 140 -
Additional sequence information
Full-length mature chain lacking the signal peptide, N-terminal glycine.
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Specifications
Our Abpromise guarantee covers the use of ab259406 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
HPLC
Functional Studies
Mass Spectrometry
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at Room Temperature. Store at Room Temperature.
pH: 6.00
Constituents: 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Monobasic dihydrogen potassium phosphate, 10.26% TrehaloseThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with phosphate buffered saline.Store lyophilized form at room temperature. Reconstitute, aliquot and store at -80°C for 12 months or +4°C for 1 week.Avoid repeated freeze-thaw.Lyophilized contents may appear as either a translucent film or a white power. This variance does not affect the quality of the product. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.
General Info
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Alternative names
- B cell growth factor 1
- B cell IgG differentiation factor
- B Cell Stimulatory Factor 1
see all -
Function
Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. -
Involvement in disease
Genetic variations in IL4 may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. -
Sequence similarities
Belongs to the IL-4/IL-13 family. -
Cellular localization
Secreted. - Information by UniProt
Images
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Functional Studies - Recombinant human IL-4 protein (Active) (ab259406)
Fully biologically active determined by dose dependent increase in HT-2 cell proliferation.
ED50 is ≤1.04 ng/ml, corresponding to a specific activity of 0.96 x 106 units/mg.
Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.
Lot: GR3372567-1
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SDS-PAGE - Recombinant human IL-4 protein (Active) (ab259406)SDS-PAGE analysis of ab259406.
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Mass Spectrometry - Recombinant human IL-4 protein (Active) (ab259406)Detarmination of mass by ESI TOF.
Predicted mass is 13615.11 Da (+/- 10 Da by ESI TOF). Observed mass is 13616.32 Da (and + 80 Da due to phosphorylation).
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HPLC - Recombinant human IL-4 protein (Active) (ab259406)HPLC analysis of ab259406.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab259406 has not yet been referenced specifically in any publications.