Overview

Description

  • Nature

    Recombinant
  • Source

    HEK 293 cells
  • Amino Acid Sequence
    • Accession
    • Species

      Mouse
    • Sequence

      IKVLGEPTCFSDYIRTSTCEWFLDSAVDCSSQLCLHYRLMFFEFSENLTC IPRNSASTVC VCHMEMNRPVQSDRYQMELWAEHRQLWQGSFSPSGNVK PLAPDNLTLHTNVSDEWLLTWN NLYPSNNLLYKDLISMVNISREDNPA EFIVYNVTYKEPRLSFPINILMSGVYYTARVRVR SQILTGTWSEWSPS ITWYNHFQLPLIQR
    • Molecular weight

      26 kDa including tags
    • Amino acids

      26 to 233
    • Tags

      His tag C-Terminus
    • Additional sequence information

      Extracellular domain (NP_001008700).

Specifications

Our Abpromise guarantee covers the use of ab221203 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Endotoxin level

    < 1.000 Eu/µg
  • Purity

    > 95 % SDS-PAGE.

  • Form

    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 7.4
    Constituents: 95% PBS, 5% Trehalose

    Lyophilized from 0.22 µm filtered solution.

  • Reconstitution
    It is recommended to reconstitute the lyophilized product in sterile deionized water to a final concentration of 1 mg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.

General Info

  • Alternative names

    • CD124
    • IL 4R alpha
    • IL-4 receptor subunit alpha
    • IL-4-binding protein
    • IL-4R subunit alpha
    • IL-4R-alpha
    • IL-4RA
    • IL4-BP
    • Il4r
    • IL4R nirs variant 1
    • IL4RA
    • IL4RA_HUMAN
    • Interleukin 4 receptor
    • Interleukin 4 receptor alpha chain
    • sIL4Ralpha/prot
    • Soluble IL-4 receptor subunit alpha
    • Soluble IL-4R-alpha
    • Soluble interleukin-4 receptor subunit alpha
    see all
  • Function

    Receptor for both interleukin 4 and interleukin 13. Couples to the JAK1/2/3-STAT6 pathway. The IL4 response is involved in promoting Th2 differentiation. The IL4/IL13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammmation. In certain cell types, can signal through activation of insulin receptor substrates, IRS1/IRS2.
    Soluble IL4R (sIL4R) inhibits IL4-mediated cell proliferation and IL5 up-regulation by T-cells.
  • Tissue specificity

    Isoform 1 and isoform 2 are highly expressed in activated T-cells.
  • Sequence similarities

    Belongs to the type I cytokine receptor family. Type 4 subfamily.
    Contains 1 fibronectin type-III domain.
  • Domain

    The extracellular domain represents the IL4 binding protein (IL4BP).
    The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.
    Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
  • Post-translational
    modifications

    On IL4 binding, phosphorylated on C-terminal tyrosine residues. Phosphorylation on any one of tyrosine residues, Tyr-575, Tyr-603 or Tyr-631, is required for STAT6-induced gene induction.
    The soluble form (sIL4R/IL4BP) can also be produced by proteolytic cleavage at the cell surface (shedding) by a metalloproteinase.
  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt

Images

  • SDS-PAGE analysis of reduced ab221203 stained overnight with Coomassie Blue.

    The reducing protein migrates as 37-45 kDa in SDS-PAGE.

References

ab221203 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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