The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
The ED50 as determined by the dose-dependent stimulation of the proliferation of murine D10S cells was found to be < 0.01ng/ml, corresponding to a specific activity of 1 x 108 IU/mg.
% SDS-PAGE. Purity: > 98% by SDS-PAGE and HPLC analysis.
Endotoxin level is <0.1 ng per µg of IL1 alpha.
ab119727 is best-stored desiccated.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).
Constituent: 99% PBS
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of not less than 100 µg/ml. which can then be diluted into other aqueous solutions.
IL 1 alpha
Interleukin 1 alpha
Preinterleukin 1 alpha
Pro interleukin 1 alpha
Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
Belongs to the IL-1 family.
The similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function.
Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.