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Bioactive grade

Recombinant mouse IL36 beta/IL-1F8 protein (Active) (ab241515)

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SDS-PAGE - Recombinant mouse IL36 beta/IL-1F8 protein (Active) (ab241515)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 97% SDS-PAGE
    • Endotoxin level: < 1.000 Eu/µg
    • Active: Yes
    • Suitable for: Functional Studies, SDS-PAGE, HPLC

    Description

    • Product name

      Recombinant mouse IL36 beta/IL-1F8 protein (Active)
      See all IL36 beta/IL-1F8 proteins and peptides
    • Biological activity

      Fully biologically active when compared to standard. The ED50 as determined by inducing IL-6 secretion in murine NIH/3T3 cells is less than 10 ng/ml, corresponding to a specific activity of >1.0x105 IU/mg.

    • Purity

      > 97 % SDS-PAGE.
      > 97 % by HPLC.
    • Endotoxin level

      < 1.000 Eu/µg
    • Expression system

      Escherichia coli
    • Accession

      Q9D6Z6
    • Protein length

      Full length protein
    • Animal free

      No
    • Nature

      Recombinant
      • Species

        Mouse
      • Sequence

        SSQSPRNYRVHDSQQMVWVLTGNTLTAVPASNNVKPVILSLIACRDTEFQ DVKKGNLVFLGIKNRNLCFCCVEMEGKPTLQLKEVDIMNLYKERKAQKAF LFYHGIEGSTSVFQSVLYPGWFIATSSIERQTIILTHQRGKLVNTNFYIE SEK
      • Predicted molecular weight

        17 kDa
      • Amino acids

        31 to 183
      • Additional sequence information

        This product is the mature full length protein from aa 31 to 183. The propeptide is not included.

    Specifications

    Our Abpromise guarantee covers the use of ab241515 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Functional Studies

      SDS-PAGE

      HPLC

    • Form

      Lyophilized
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      Constituents: PBS, 5% Trehalose

      0.2 µm filtered.

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    • Reconstitution
      We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at = -20 °C. Further dilutions should be made in appropriate buffered solutions.

    General Info

    • Alternative names

      • 2310043N20Rik
      • Family of interleukin 1 eta
      • FIL1
      • FIL1 (ETA)
      • FIL1 eta
      • FIL1H
      • FILI-(ETA)
      • IL 1F8
      • IL 1F8 (FIL1 eta
      • IL 1H2
      • IL-1 eta
      • IL-1F8
      • IL-1H2
      • IL1 ETA
      • IL1F8
      • IL1F8 (Canonical product IL 1F8a)
      • IL1F8_HUMAN
      • IL1H2
      • IL36 beta
      • IL36B
      • Interleukin 1 family, member 8
      • Interleukin 1 family, member 8 (eta)
      • Interleukin 1 homolog 2
      • Interleukin 1 Superfamily e
      • Interleukin 36 beta
      • Interleukin-1 eta
      • Interleukin-1 family member 8
      • Interleukin-1 homolog 2
      • MGC126880
      • MGC126882
      • OTTMUSP00000012797
      • RP23-176J12.1
      see all
    • Tissue specificity

      Expression at low levels in tonsil, bone marrow, heart, placenta, lung, testis and colon but not in other tissues or in any hematopoietic cell lines.
    • Sequence similarities

      Belongs to the IL-1 family.
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession Q9NZH7 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    Images

    • SDS-PAGE - Recombinant mouse IL36 beta/IL-1F8 protein (Active) (ab241515)
      SDS-PAGE - Recombinant mouse IL36 beta/IL-1F8 protein (Active) (ab241515)

      SDS-PAGE analysis of ab241515.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab241515? Please let us know so that we can cite the reference in this datasheet.

    ab241515 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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