Recombinant mouse IL36 gamma/IL-1F9 protein (Active) (ab241519)
Key features and details
- Expression system: Escherichia coli
- Purity: > 98% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE, HPLC
Description
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Product name
Recombinant mouse IL36 gamma/IL-1F9 protein (Active)
See all IL36 gamma/IL-1F9 proteins and peptides -
Biological activity
Fully biologically active when compared to standard. The ED50 as determined by inducing IL-6 secretion in murine NIH/3T3 cells is less than 10 ng/ml, corresponding to a specific activity of >1.0 x 105 IU/mg.
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Purity
> 98 % SDS-PAGE.
Purity >98% as determined by HPLC -
Endotoxin level
< 1.000 Eu/µg -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Mouse -
Sequence
GRETPDFGEVFDLDQQVWIFRNQALVTVPRSHRVTPVSVTILPCKYPESL EQDKGIAIYLGIQNPDKCLFCKEVNGHPTLLLKEEKILDLYHHPEPMKPF LFYHTRTGGTSTFESVAFPGHYIASSKTGNPIFLTSKKGEYYNINFNLDI KS -
Predicted molecular weight
17 kDa -
Amino acids
13 to 164 -
Additional sequence information
Full-length mature chain lacking the pro-peptide.
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Specifications
Our Abpromise guarantee covers the use of ab241519 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
HPLC
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituents: 0.32% Tris HCl, 0.87% Sodium chloride, 0.02% Tween
0.2 um filteredThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionWe recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at = -20 °C. Further dilutions should be made in appropriate buffered solutions.
General Info
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Alternative names
- IL 1 epsilon
- IL 1 related protein 2
- IL 1(EPSILON)
see all -
Function
Function as an agonist of NF-kappa B activation through the orphan IL-1-receptor-related protein 2. Could constitute part of an independent signaling system analogous to interleukin-1 alpha (IL-1A), beta (IL-1B) receptor agonist and interleukin-1 receptor type I (IL-1R1), that is present in epithelial barriers and takes part in local inflammatory response. -
Tissue specificity
Highly expressed in tissues containing epithelial cells: skin, lung, stomach and esophagus. In skin is expressed only in keratinocytes but not in fibroblasts, endothelial cells or melanocytes. Up-regulated in lesional psoriasis skin. -
Sequence similarities
Belongs to the IL-1 family. -
Cellular localization
Secreted. - Information by UniProt
Images
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SDS-PAGE - Recombinant mouse IL36 gamma/IL-1F9 protein (Active) (ab241519)
SDS-PAGE - Recombinant mouse IL-36 gamma/IL-1F9 protein (Active) (ab241519)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab241519 has not yet been referenced specifically in any publications.
