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Bioactive grade

Recombinant mouse IL36 gamma/IL-1F9 protein (Active) (ab241519)

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SDS-PAGE - Recombinant mouse IL36 gamma/IL-1F9 protein (Active) (ab241519)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 98% SDS-PAGE
    • Endotoxin level: < 1.000 Eu/µg
    • Active: Yes
    • Suitable for: Functional Studies, SDS-PAGE, HPLC

    Description

    • Product name

      Recombinant mouse IL36 gamma/IL-1F9 protein (Active)
      See all IL36 gamma/IL-1F9 proteins and peptides
    • Biological activity

      Fully biologically active when compared to standard. The ED50 as determined by inducing IL-6 secretion in murine NIH/3T3 cells is less than 10 ng/ml, corresponding to a specific activity of >1.0 x 105 IU/mg.

    • Purity

      > 98 % SDS-PAGE.
      Purity >98% as determined by HPLC
    • Endotoxin level

      < 1.000 Eu/µg
    • Expression system

      Escherichia coli
    • Accession

      Q8R460
    • Protein length

      Full length protein
    • Animal free

      No
    • Nature

      Recombinant
      • Species

        Mouse
      • Sequence

        GRETPDFGEVFDLDQQVWIFRNQALVTVPRSHRVTPVSVTILPCKYPESL EQDKGIAIYLGIQNPDKCLFCKEVNGHPTLLLKEEKILDLYHHPEPMKPF LFYHTRTGGTSTFESVAFPGHYIASSKTGNPIFLTSKKGEYYNINFNLDI KS
      • Predicted molecular weight

        17 kDa
      • Amino acids

        13 to 164
      • Additional sequence information

        Full-length mature chain lacking the pro-peptide.

    Specifications

    Our Abpromise guarantee covers the use of ab241519 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Functional Studies

      SDS-PAGE

      HPLC

    • Form

      Lyophilized
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      Constituents: 0.32% Tris HCl, 0.87% Sodium chloride, 0.02% Tween

      0.2 um filtered

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    • Reconstitution
      We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at = -20 °C. Further dilutions should be made in appropriate buffered solutions.

    General Info

    • Alternative names

      • IL 1 epsilon
      • IL 1 related protein 2
      • IL 1(EPSILON)
      • IL 1F9
      • IL 1H1
      • IL 1RP2
      • IL-1 epsilon
      • IL-1-related protein 2
      • IL-1F9
      • IL-1H1
      • IL-1RP2
      • IL1E
      • Il1f9
      • IL1F9_HUMAN
      • IL1H1
      • IL1RP2
      • IL36 gamma
      • IL36G
      • Interleukin 1 epsilon
      • Interleukin 1 family member 9
      • Interleukin 1 homolog 1
      • Interleukin 1 related protein 2
      • Interleukin 36 gamma
      • Interleukin-1 epsilon
      • Interleukin-1 family member 9
      • Interleukin-1 homolog 1
      see all
    • Function

      Function as an agonist of NF-kappa B activation through the orphan IL-1-receptor-related protein 2. Could constitute part of an independent signaling system analogous to interleukin-1 alpha (IL-1A), beta (IL-1B) receptor agonist and interleukin-1 receptor type I (IL-1R1), that is present in epithelial barriers and takes part in local inflammatory response.
    • Tissue specificity

      Highly expressed in tissues containing epithelial cells: skin, lung, stomach and esophagus. In skin is expressed only in keratinocytes but not in fibroblasts, endothelial cells or melanocytes. Up-regulated in lesional psoriasis skin.
    • Sequence similarities

      Belongs to the IL-1 family.
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession Q9NZH8 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    Images

    • SDS-PAGE - Recombinant mouse IL36 gamma/IL-1F9 protein (Active) (ab241519)
      SDS-PAGE - Recombinant mouse IL36 gamma/IL-1F9 protein (Active) (ab241519)

      SDS-PAGE - Recombinant mouse IL-36 gamma/IL-1F9 protein (Active) (ab241519)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab241519? Please let us know so that we can cite the reference in this datasheet.

    ab241519 has not yet been referenced specifically in any publications.

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