Bioactive grade

Recombinant mouse IL5 protein (Active) (Animal Free) (ab218100)

Description

  • Product name

    Recombinant mouse IL5 protein (Active) (Animal Free)
    See all IL-5 proteins and peptides
  • Biological activity

    Determined by TF-1 cell proliferation assay. ED50 ≤2 ng/mL; ≥ 5.0 x 105 units/mg (approximate units).

  • Purity

    > 95 % SDS-PAGE.

  • Endotoxin level

    < 1.000 Eu/µg
  • Expression system

    Escherichia coli
  • Accession

  • Protein length

    Full length protein
  • Animal free

    Yes
  • Nature

    Recombinant
    • Species

      Mouse
    • Sequence

      MEIPMSTVVK ETLTQLSAHR ALLTSNETMR LPVPTHKNHQ LCIGEIFQGL DILKNQTVRG GTVEMLFQNL SLIKKYIDRQ KEKCGEERRR TRQFLDYLQE FLGVMSTEWA MEG
    • Predicted molecular weight

      13 kDa
    • Amino acids

      21 to 133
    • Additional sequence information

      This product is for the mature full length protein. The signal peptide is not included.
  • Description

    Recombinant mouse IL-5 protein (Active)

Specifications

Our Abpromise guarantee covers the use of ab218100 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

  • Form

    Lyophilised
  • Additional notes

    This product is produced with no animal-derived raw products. All processing and handling employs animal-free equipment and animal-free protocols.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at Room Temperature. Store at -20°C or -80°C.

    pH: 9.00
    Constituent: 0.17% Sodium bicarbonate

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Reconstitute in sterile water at 0.1 mg/mL. Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.

General Info

  • Alternative names

    • B-cell differentiation factor I
    • Colony stimulating factor
    • EDF
    • Eosinophil differentiation factor
    • IL-5
    • IL5
    • IL5_HUMAN
    • Interleukin 5
    • Interleukin 5 (colony stimulating factor, eosinophil)
    • Interleukin-5
    • T Cell Replacing Factor
    • T-cell replacing factor
    • TRF
    see all
  • Function

    Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells.
  • Sequence similarities

    Belongs to the IL-5 family.
  • Cellular localization

    Secreted.
  • Information by UniProt

Images

  • SDS-PAGE analysis of ab218100 (1μg) stained with Coomassie blue:

    Lane 1: Non-reduced

    Lane 2: Reduced

    4-20% Tris-Glycine gel

References

ab218100 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab218100.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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