Recombinant mouse Jagged1 protein (Fc Chimera Active) (ab109346)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 90% SDS-PAGE
- Endotoxin level: < 0.010 Eu/µg
- Active: Yes
- Tags: Fc tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies, Inhibition Assay
Description
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Product name
Recombinant mouse Jagged1 protein (Fc Chimera Active)
See all Jagged1 proteins and peptides -
Biological activity
Inhibits adipogenesis in NIH/3T3 cells.
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Purity
> 90 % SDS-PAGE.
ab109346 is lyophilised from a 0.2 µm filtered solution. Purified using affinity chromatography. -
Endotoxin level
< 0.010 Eu/µg -
Expression system
HEK 293 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Mouse -
Predicted molecular weight
150 kDa including tags -
Amino acids
1 to 1067 -
Tags
Fc tag C-Terminus -
Additional sequence information
Signal peptide and extracellular domain of mouse Jagged-1 (aa 1-1067) are fused at the C-terminus to the Fc portion of human IgG.
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Specifications
Our Abpromise guarantee covers the use of ab109346 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
Inhibition Assay
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Constituent: PBS
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionFor 10µg size: reconstitute with 100µl sterile water. For 50µg size: reconstitute with 50µl sterile water
General Info
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Alternative names
- AGS
- AHD
- AWS
see all -
Function
Ligand for multiple Notch receptors and involved in the mediation of Notch signaling. May be involved in cell-fate decisions during hematopoiesis. Seems to be involved in early and late stages of mammalian cardiovascular development. Inhibits myoblast differentiation (By similarity). Enhances fibroblast growth factor-induced angiogenesis (in vitro). -
Tissue specificity
Widely expressed in adult and fetal tissues. In cervix epithelium expressed in undifferentiated subcolumnar reserve cells and squamous metaplasia. Expression is up-regulated in cervical squamous cell carcinoma. Expressed in bone marrow cell line HS-27a which supports the long-term maintenance of immature progenitor cells. -
Involvement in disease
Defects in JAG1 are the cause of Alagille syndrome type 1 (ALGS1) [MIM:118450]. Alagille syndrome is an autosomal dominant multisystem disorder defined clinically by hepatic bile duct paucity and cholestasis in association with cardiac, skeletal, and ophthalmologic manifestations. There are characteristic facial features and less frequent clinical involvement of the renal and vascular systems.
Defects in JAG1 are a cause of tetralogy of Fallot (TOF) [MIM:187500]. TOF is a congenital heart anomaly which consists of pulmonary stenosis, ventricular septal defect, dextroposition of the aorta (aorta is on the right side instead of the left) and hypertrophy of the right ventricle. This condition results in a blue baby at birth due to inadequate oxygenation. Surgical correction is emergent. -
Sequence similarities
Contains 1 DSL domain.
Contains 15 EGF-like domains. -
Developmental stage
Expressed in 32-52 days embryos in the distal cardiac outflow tract and pulmonary artery, major arteries, portal vein, optic vesicle, otocyst, branchial arches, metanephros, pancreas, mesocardium, around the major bronchial branches, and in the neural tube. -
Cellular localization
Membrane. - Information by UniProt
Images
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Inhibition Assay - Recombinant mouse Jagged1 protein (Fc Chimera Active) (ab109346)
Differentiation of 3T3-L1 cells was performed by first growing cells to confluence (day 2) and then shifting them to differentiation medium after 48 h (day 0). The differentiation medium contained 1 μM dexamethasone, 0.5 mM IBMX, and 15μg/ml of insulin. Differentiation medium lacking dexamethasone and IBMX and addition of mouse TNF-alpha (20mg/ml) were both used as a control. As a negative control hCD137-Fc was used. Medium was changed to growth medium containing only insulin (15μg/ml) after 48 h (day 2). Staining with Oil Red O was typically performed on day 7. Notch signaling was generated by immobilizing recombinant mouse Jagged-1-Fc on plates (Jagged-1 was coated on 12-well plate at 5µg/ml (1µg/ml should also work) overnight at 4°C). These plates (with or without Notch ligand) were then used to grow 3T3-L1 cells.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (1)
ab109346 has been referenced in 1 publication.
- Zhu P et al. Curcumin attenuates hypoxia/reoxygenation-induced cardiomyocyte injury by downregulating Notch signaling. Mol Med Rep 20:1541-1550 (2019). PubMed: 31257466
