For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Explore the power of knock-out cell lines for your research

  1. Link

    recombinant-mouse-jagged1-protein-fc-chimera-active-ab109346.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Cardiovascular Angiogenesis Inhibitors
Share by email
Bioactive grade

Recombinant mouse Jagged1 protein (Fc Chimera Active) (ab109346)

  • Datasheet
Submit a review Submit a question References (1)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Inhibition Assay - Recombinant mouse Jagged1 protein (Fc Chimera Active) (ab109346)

    Key features and details

    • Expression system: HEK 293 cells
    • Purity: > 90% SDS-PAGE
    • Endotoxin level: < 0.010 Eu/µg
    • Active: Yes
    • Tags: Fc tag C-Terminus
    • Suitable for: SDS-PAGE, Functional Studies, Inhibition Assay

    You may also be interested in

    Primary
    Product image
    Anti-Collagen VI alpha 2 antibody [EPR7889(N)] (ab180855)
    Primary
    Product image
    Anti-PTPRD antibody [5H5D7] (ab233806)
    Protein
    Recombinant human Wnt1 protein (Active) (ab84080)

    View more associated products

    Description

    • Product name

      Recombinant mouse Jagged1 protein (Fc Chimera Active)
      See all Jagged1 proteins and peptides
    • Biological activity

      Inhibits adipogenesis in NIH/3T3 cells.

    • Purity

      > 90 % SDS-PAGE.
      ab109346 is lyophilised from a 0.2 µm filtered solution. Purified using affinity chromatography.
    • Endotoxin level

      < 0.010 Eu/µg
    • Expression system

      HEK 293 cells
    • Accession

      Q9QXX0
    • Protein length

      Protein fragment
    • Animal free

      No
    • Nature

      Recombinant
      • Species

        Mouse
      • Predicted molecular weight

        150 kDa including tags
      • Amino acids

        1 to 1067
      • Tags

        Fc tag C-Terminus
      • Additional sequence information

        Signal peptide and extracellular domain of mouse Jagged-1 (aa 1-1067) are fused at the C-terminus to the Fc portion of human IgG.

    Specifications

    Our Abpromise guarantee covers the use of ab109346 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      Functional Studies

      Inhibition Assay

    • Form

      Lyophilized
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

      Constituent: PBS

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    • Reconstitution
      For 10µg size: reconstitute with 100µl sterile water. For 50µg size: reconstitute with 50µl sterile water

    General Info

    • Alternative names

      • AGS
      • AHD
      • AWS
      • CD 339
      • CD339
      • CD339 antigen
      • Headturner
      • hJ1
      • Htu
      • Jag 1
      • Jag1
      • JAG1_HUMAN
      • Jagged 1
      • Jagged1
      • Jagged1 (Alagille syndrome)
      • JAGL1
      • MGC104644
      • OTTHUMP00000030278
      • Protein jagged-1
      • Ser 1
      • Ser1
      • Serrate 1
      • Slalom
      see all
    • Function

      Ligand for multiple Notch receptors and involved in the mediation of Notch signaling. May be involved in cell-fate decisions during hematopoiesis. Seems to be involved in early and late stages of mammalian cardiovascular development. Inhibits myoblast differentiation (By similarity). Enhances fibroblast growth factor-induced angiogenesis (in vitro).
    • Tissue specificity

      Widely expressed in adult and fetal tissues. In cervix epithelium expressed in undifferentiated subcolumnar reserve cells and squamous metaplasia. Expression is up-regulated in cervical squamous cell carcinoma. Expressed in bone marrow cell line HS-27a which supports the long-term maintenance of immature progenitor cells.
    • Involvement in disease

      Defects in JAG1 are the cause of Alagille syndrome type 1 (ALGS1) [MIM:118450]. Alagille syndrome is an autosomal dominant multisystem disorder defined clinically by hepatic bile duct paucity and cholestasis in association with cardiac, skeletal, and ophthalmologic manifestations. There are characteristic facial features and less frequent clinical involvement of the renal and vascular systems.
      Defects in JAG1 are a cause of tetralogy of Fallot (TOF) [MIM:187500]. TOF is a congenital heart anomaly which consists of pulmonary stenosis, ventricular septal defect, dextroposition of the aorta (aorta is on the right side instead of the left) and hypertrophy of the right ventricle. This condition results in a blue baby at birth due to inadequate oxygenation. Surgical correction is emergent.
    • Sequence similarities

      Contains 1 DSL domain.
      Contains 15 EGF-like domains.
    • Developmental stage

      Expressed in 32-52 days embryos in the distal cardiac outflow tract and pulmonary artery, major arteries, portal vein, optic vesicle, otocyst, branchial arches, metanephros, pancreas, mesocardium, around the major bronchial branches, and in the neural tube.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession P78504 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    Images

    • Inhibition Assay - Recombinant mouse Jagged1 protein (Fc Chimera Active) (ab109346)
      Inhibition Assay - Recombinant mouse Jagged1 protein (Fc Chimera Active) (ab109346)

      Differentiation of 3T3-L1 cells was performed by first growing cells to confluence (day 2) and then shifting them to differentiation medium after 48 h (day 0). The  differentiation medium contained 1 μM dexamethasone, 0.5 mM IBMX, and 15μg/ml of insulin. Differentiation medium lacking dexamethasone and IBMX and addition of mouse TNF-alpha (20mg/ml) were both used as a control. As a negative control hCD137-Fc was used. Medium was changed to growth medium containing only insulin (15μg/ml) after 48 h (day 2). Staining with Oil Red O was typically performed on day 7. Notch signaling was generated by immobilizing recombinant mouse Jagged-1-Fc on plates (Jagged-1 was coated on 12-well plate at 5µg/ml (1µg/ml should also work) overnight at 4°C). These plates (with or without Notch ligand) were then used to grow 3T3-L1 cells.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (1)

    Publishing research using ab109346? Please let us know so that we can cite the reference in this datasheet.

    ab109346 has been referenced in 1 publication.

    • Zhu P  et al. Curcumin attenuates hypoxia/reoxygenation-induced cardiomyocyte injury by downregulating Notch signaling. Mol Med Rep 20:1541-1550 (2019). PubMed: 31257466

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab109346.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2022 Abcam plc. All rights reserved.