Bioactive grade

Recombinant mouse LDL Receptor protein (ab206024)

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Functional Studies - Recombinant mouse LDL Receptor protein (ab206024)
  • SDS-PAGE - Recombinant mouse LDL Receptor protein (ab206024)

Key features and details

  • Expression system: HEK 293 cells
  • Purity: > 95% SDS-PAGE
  • Endotoxin level: < 1.000 Eu/µg
  • Active: Yes
  • Tags: His tag C-Terminus
  • Suitable for: SDS-PAGE, ELISA, Functional Studies

Description

  • Product name

    Recombinant mouse LDL Receptor protein
    See all LDL Receptor proteins and peptides

  • Biological activity

    Measured by its binding ability in a functional ELISA. Immobilized ab206024 at 10 μg/mL (100 μL/well) can bind Biotinylated Mouse PCSK9 with a linear range of 2-16 ng/mL.

  • Purity

    > 95 % SDS-PAGE.

  • Endotoxin level

    < 1.000 Eu/µg

  • Expression system

    HEK 293 cells

  • Accession

    P35951

  • Protein length

    Protein fragment

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Mouse

    • Sequence

      AAEDSCSRNEFQCRDGKCIASKWVCDGSPECPDGSDESPETCMSVTCQSN QFSCGGRVSRCIPDSWRCDGQVDCENDSDEQGCPPKTCSQDDFRCQDGKC ISPQFVCDGDRDCLDGSDEAHCQATTCGPAHFRCNSSICIPSLWACDGDV DCVDGSDEWPQNCQGRDTASKGVSSPCSSLEFHCGSSECIHRSWVCDGEA DCKDKSDEEHCAVATCRPDEFQCADGSCIHGSRQCDREHDCKDMSDELGC VNVTQCDGPNKFKCHSGECISLDKVCDSARDCQDWSDEPIKECKTNECLD NNGGCSHICKDLKIGSECLCPSGFRLVDLHRCEDIDECQEPDTCSQLCVN LEGSYKCECQAGFHMDPHTRVCKAVGSIGYLLFTNRHEVRKMTLDRSEYT SLLPNLKNVVALDTEVTNNRIYWSDLSQKKIYSALMDQAPNLSYDTIISE DLHAPDGLAVDWIHRNIYWTDSVPGSVSVADTKGVKRRTLFQEAGSRPRA IVVDPVHGFMYWTDWGTPAKIKKGGLNGVDIHSLVTENIQWPNGITLDLS SGRLYWVDSKLHSISSIDVNGGNRKTILEDENRLAHPFSLAIYEDKVYWT DVINEAIFSANRLTGSDVNLVAENLLSPEDIVLFHKVTQPRGVNWCETTA LLPNGGCQYLCLPAPQIGPHSPKFTCACPDGMLLAKDMRSCLTEVDTVLT TQGTSAVRPVVTASATRPPKHSEDLSAPSTPRQPVDTPGLSTVASVTVSH QVQGDMAGRGNEEQPHGMR

    • Predicted molecular weight

      86 kDa including tags

    • Amino acids

      22 to 790

    • Tags

      His tag C-Terminus

    • Additional sequence information

      NP_034830.

Specifications

Our Abpromise guarantee covers the use of ab206024 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    ELISA

    Functional Studies

  • Form

    Lyophilized

  • Additional notes

    This product is stable after storage at:
    • -20°C to -70°C for 12 months in lyophilized state;
    • -70°C for 3 months under sterile conditions after reconstitution.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Please see notes section.

    pH: 7.40
    Constituent: 100% PBS

    5-10% trehalose is commonly used for freeze drying, and after reconstitution, the trehalose is mostly about 3-5%

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Reconstitute with sterile deionized water to a concentration of 200 µg/ml.

General Info

  • Alternative names

    • FH
    • FHC
    • LDL R
    • LDL receptor
    • LDLCQ2
    • Ldlr
    • LDLR_HUMAN
    • Low Density Lipoprotein Receptor
    • Low density lipoprotein receptor class A domain containing protein 3
    • Low density lipoprotein receptor familial hypercholesterolemia
    • Low-density lipoprotein receptor
    see all

  • Function

    Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. In case of HIV-1 infection, functions as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells.

  • Involvement in disease

    Defects in LDLR are the cause of familial hypercholesterolemia (FH) [MIM:143890]; a common autosomal semi-dominant disease that affects about 1 in 500 individuals. The receptor defect impairs the catabolism of LDL, and the resultant elevation in plasma LDL-cholesterol promotes deposition of cholesterol in the skin (xanthelasma), tendons (xanthomas), and coronary arteries (atherosclerosis).

  • Sequence similarities

    Belongs to the LDLR family.
    Contains 3 EGF-like domains.
    Contains 7 LDL-receptor class A domains.
    Contains 6 LDL-receptor class B repeats.

  • Post-translational
    modifications

    N- and O-glycosylated.
    Ubiquitinated by MYLIP leading to degradation.

  • Cellular localization

    Cell membrane. Endomembrane system. Membrane > clathrin-coated pit. Found distributed from the plasma membrane to intracellular compartments.
  • Information by UniProt

Images

  • Functional Studies - Recombinant mouse LDL Receptor protein (ab206024)
    Functional Studies - Recombinant mouse LDL Receptor protein (ab206024)

    Immobilized ab206024 at 10 μg/mL (100 μL/well) can bind Biotinylated Mouse PCSK9 with a linear range of 2-16 ng/mL.

  • SDS-PAGE - Recombinant mouse LDL Receptor protein (ab206024)
    SDS-PAGE - Recombinant mouse LDL Receptor protein (ab206024)

    The gel was stained overnight with Coomassie Blue. The protein migrates as 120 kDa under reducing (R) condition due to glycosylation.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab206024? Please let us know so that we can cite the reference in this datasheet.

ab206024 has not yet been referenced specifically in any publications.

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