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Bioactive grade

Recombinant mouse liver FABP protein (Active) (ab243222)

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SDS-PAGE - Recombinant mouse liver FABP protein (Active) (ab243222)

    Key features and details

    • Expression system: Escherichia coli
    • Endotoxin level: < 1.000 Eu/µg
    • Active: Yes
    • Suitable for: Functional Studies, HPLC, SDS-PAGE

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    Mouse Liver-FABP Matched Antibody Pair Kit (ab220137)

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    Description

    • Product name

      Recombinant mouse liver FABP protein (Active)
      See all liver FABP proteins and peptides
    • Biological activity

      Fully biologically active when compared to standard. The binding affinity of rMuFABP1 for the synthetic ligand cis-parinaric acid has been measured by fluorescence titration. Half maximal fluorescence of 2.5 μM rMuFABP1 is achieved with approximately 5 μM cis-paranaric acid.

    • Endotoxin level

      < 1.000 Eu/µg
    • Expression system

      Escherichia coli
    • Accession

      P12710
    • Protein length

      Full length protein
    • Animal free

      No
    • Nature

      Recombinant
      • Species

        Mouse
      • Sequence

        MNFSGKYQLQSQENFEPFMKAIGLPEDLIQKGKDIKGVSEIVHEGKKIKL TITYGPKVVRNEFTLGEECELETMTGEKVKAVVKLEGDNKMVTTFKGIKS VTELNGDTITNTMTLGDIVYKRVSKRI
      • Predicted molecular weight

        14 kDa
      • Amino acids

        1 to 127

    Associated products

      Specifications

      Our Abpromise guarantee covers the use of ab243222 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      • Applications

        Functional Studies

        HPLC

        SDS-PAGE

      • Form

        Lyophilized
      • Concentration information loading...

      Preparation and Storage

      • Stability and Storage

        Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

        Constituent: 0.87% Sodium chloride

        20 mM Phosphate buffer. 0.2 micron filtered.

        This product is an active protein and may elicit a biological response in vivo, handle with caution.

      • Reconstitution
        Briefly centrifuge prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20 °C or below. Further dilutions should be made in appropriate buffered solutions.

      General Info

      • Alternative names

        • FABP 1
        • FABP1
        • FABPL
        • FABPL_HUMAN
        • Fatty Acid Binding Protein
        • Fatty Acid Binding Protein 1
        • Fatty acid binding protein 1 liver
        • Fatty acid-binding protein
        • Fatty acid-binding protein 1
        • Fatty acid-binding protein liver
        • L FABP
        • L-FABP
        • liver
        • Liver-type fatty acid-binding protein
        see all
      • Function

        Binds free fatty acids and their coenzyme A derivatives, bilirubin, and some other small molecules in the cytoplasm. May be involved in intracellular lipid transport.
      • Sequence similarities

        Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
      • Domain

        Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior.
      • Cellular localization

        Cytoplasm.
      • Target information above from: UniProt accession P07148 The UniProt Consortium
        The Universal Protein Resource (UniProt) in 2010
        Nucleic Acids Res. 38:D142-D148 (2010) .

        Information by UniProt

      Images

      • SDS-PAGE - Recombinant mouse liver FABP protein (Active) (ab243222)
        SDS-PAGE - Recombinant mouse liver FABP protein (Active) (ab243222)
        SDS Page analysis of ab243222

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab243222? Please let us know so that we can cite the reference in this datasheet.

      ab243222 has not yet been referenced specifically in any publications.

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