Recombinant mouse M-CSF protein (ab129146)
Key features and details
- Expression system: Escherichia coli
- Purity: >= 95% SDS-PAGE
- Endotoxin level: <= 1.000 Eu/µg
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
Description
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Product name
Recombinant mouse M-CSF protein
See all M-CSF proteins and peptides -
Biological activity
Method of Determination: NFS-60 cell proliferation
Acception Criteria: ED50 ≤10 ng/mL(≥ 1.0 x 10^5 units/mg)
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Purity
>= 95 % SDS-PAGE.
Purified using standard chromatographical techniques. -
Endotoxin level
<=1.000 Eu/µg -
Expression system
Escherichia coli -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Mouse -
Sequence
MKEVSEHCSH MIGNGHLKVL QQLIDSQMET SCQIAFEFVD QEQLDDPVCY LKKAFFLVQD IIDETMRFKD NTPNANATER LQELSNNLNS CFTKDYEEQN KACVRTFHET PLQLLEKIKN FFNETKNLLE KDWNIFTKNC NNSFAKCSSR DVVTKPMKEV SEHCSHMIGN GHLKVLQQLI DSQMETSCQI AFEFVDQEQL DDPVCYLKKA FFLVQDIIDE TMRFKDNTPN ANATERLQEL SNNLNSCFTK DYEEQNKACV RTFHETPLQL LEKIKNFFNE TKNLLEKDWN IFTKNCNNSF AKCSSRDVVT KP -
Predicted molecular weight
36 kDa -
Amino acids
33 to 187
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Specifications
Our Abpromise guarantee covers the use of ab129146 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
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Form
Lyophilized -
Additional notes
Endotoxin levels determined by kinetic LAL (50% Confidence).
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at Room Temperature. Upon reconsitution add a carrier protein (0.1% BSA). Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.16% Sodium phosphate, 0.29% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionSterile water at 0.1 mg/mL Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution.
General Info
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Alternative names
- Colony stimulating factor 1
- Colony stimulating factor 1 (macrophage)
- Colony stimulating factor macrophage specific
see all -
Function
Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. CSF-1 induces cells of the monocyte/macrophage lineage. It plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy. -
Post-translational
modificationsGlycosylation and proteolytic cleavage yield different soluble forms. A high molecular weight soluble form is a proteoglycan containing chondroitin sulfate.
Isoform 1 is N- and O-glycosylated. Isoform 3 is N-glycosylated. -
Cellular localization
Cell membrane and Secreted > extracellular space. - Information by UniProt
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (5)
ab129146 has been referenced in 5 publications.
- Bresque M et al. SIRT6 stabilization and cytoplasmic localization in macrophages regulates acute and chronic inflammation in mice. J Biol Chem 298:101711 (2022). PubMed: 35150745
- Gao Z et al. Nociceptor Neurons are Involved in the Host Response to Escherichia coli Urinary Tract Infections. J Inflamm Res 15:3337-3353 (2022). PubMed: 35702548
- Huang C et al. Hepatocyte growth factor overexpression promotes osteoclastogenesis and exacerbates bone loss in CIA mice. J Orthop Translat 27:9-16 (2021). PubMed: 33344167
- Wang R et al. Urinary Trypsin Inhibitor Protects Tight Junctions of Septic Pulmonary Capillary Endothelial Cells by Regulating the Functions of Macrophages. J Inflamm Res 14:1973-1989 (2021). PubMed: 34045879
- Zheng Y et al. Gentiana scabra Restrains Hepatic Pro-Inflammatory Macrophages to Ameliorate Non-Alcoholic Fatty Liver Disease. Front Pharmacol 12:816032 (2021). PubMed: 35115947