Overview

Description

  • Nature

    Recombinant
  • Source

    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species

      Mouse
    • Sequence

      MKEVSEHCSH MIGNGHLKVL QQLIDSQMET SCQIAFEFVD QEQLDDPVCY LKKAFFLVQD IIDETMRFKD NTPNANATER LQELSNNLNS CFTKDYEEQN KACVRTFHET PLQLLEKIKN FFNETKNLLE KDWNIFTKNC NNSFAKCSSR DVVTKPMKEV SEHCSHMIGN GHLKVLQQLI DSQMETSCQI AFEFVDQEQL DDPVCYLKKA FFLVQDIIDE TMRFKDNTPN ANATERLQEL SNNLNSCFTK DYEEQNKACV RTFHETPLQL LEKIKNFFNE TKNLLEKDWN IFTKNCNNSF AKCSSRDVVT KP
    • Molecular weight

      36 kDa
    • Amino acids

      33 to 187

Specifications

Our Abpromise guarantee covers the use of ab129146 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    Method of Determination: NFS-60 cell proliferation

    Acception Criteria: ED50 ≤10 ng/mL(≥ 1.0 x 10^5 units/mg)

  • Applications

    SDS-PAGE

    Functional Studies

  • Endotoxin level

    <=1.000 Eu/µg
  • Purity

    >= 95 % SDS-PAGE.

  • Form

    Lyophilised
  • Additional notes

    Endotoxin levels determined by kinetic LAL (50% Confidence).

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at Room Temperature. Upon reconsitution add a carrier protein (0.1% BSA). Store at -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.16% Sodium phosphate, 0.29% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Sterile water at 0.1 mg/mL Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution.

General Info

  • Alternative names

    • Colony stimulating factor 1
    • Colony stimulating factor 1 (macrophage)
    • Colony stimulating factor macrophage specific
    • CSF 1
    • CSF-1
    • CSF1
    • CSF1_HUMAN
    • Csfm
    • Lanimostim
    • M CSF
    • M-CSF
    • Macrophage colony stimulating factor
    • Macrophage Colony Stimulating Factor 1
    • MCSF
    • MGC31930
    • Processed macrophage colony-stimulating factor 1
    see all
  • Function

    Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. CSF-1 induces cells of the monocyte/macrophage lineage. It plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy.
  • Post-translational
    modifications

    Glycosylation and proteolytic cleavage yield different soluble forms. A high molecular weight soluble form is a proteoglycan containing chondroitin sulfate.
    Isoform 1 is N- and O-glycosylated. Isoform 3 is N-glycosylated.
  • Cellular localization

    Cell membrane and Secreted > extracellular space.
  • Information by UniProt

Images

  • 1 ug of ab129146 on 4-20% Tris-Glycine SDS-PAGE, stained with Coomassie Blue.

    Lane 1: Non-reducing

    Lane 2: Reducing.

  • Dose-dependent induction of M-NFS-60 cell proliferation using ab129146. Cells were incubated for 4.5 hours and then measured at 490 nm. Assays were carried out in duplicate.

References

ab129146 has not yet been referenced specifically in any publications.

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