Recombinant Mouse Mast Cell Tryptase protein (His tag) (ab236929)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, MS
Description
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Product name
Recombinant Mouse Mast Cell Tryptase protein (His tag)
See all Mast Cell Tryptase proteins and peptides -
Purity
> 90 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Mouse -
Sequence
AHGNKWPWQVSLRANDTYWMHFCGGSLIHPQWVLTAAHCVGPDVADPNKV RVQLRKQYLYYHDHLMTVSQIITHPDFYIVQDGADIALLKLTNPVNISDY VHPVPLPPASETFPSGTLCWVTGWGNIDNGVNLPPPFPLKEVQVPIIENH LCDLKYHKGLITGDNVHIVRDDMLCAGNEGHDSCQGDSGGPLVCKVEDTW LQAGVVSWGEGCAQPNRPGIYTRVTYYLDWIHH -
Predicted molecular weight
30 kDa including tags -
Amino acids
35 to 267 -
Tags
His tag N-Terminus -
Additional sequence information
N-terminal 6xHis-tagged.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab236929 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Mass Spectrometry
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Mass spectrometry
LC-MS/MS -
Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C. Avoid freeze / thaw cycle.
pH: 7.2
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
General Info
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Alternative names
- alpha II
- Lung tryptase
- Mast cell alpha II tryptase
see all -
Relevance
Mast cells contain a number of preformed chemical mediators such as histamine, chymase, carboxypeptidase and proteolytic tryptase. Human Mast Cell Tryptase is considered to be an important marker of mast cell activation as well as an important mediator of inflammation. -
Cellular localization
Secreted. Note: released from the secretory granules upon mast cell activation.
Images
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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis with 5% enrichment gel and 15% separation gel of ab236929.
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Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of ab236929 could indicate that this peptide derived from E.coli-expressed Mus musculus (Mouse) Mast Cell Tryptase.
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Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of ab236929 could indicate that this peptide derived from E.coli-expressed Mus musculus (Mouse) Mast Cell Tryptase.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab236929 has not yet been referenced specifically in any publications.