• Nature
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Species
    • Sequence
      Recombinant murine JE, also known as monocyte chemotactic protein (MCP-1), is a 13.8 kDa protein containing 125 amino acid residues. QPDAVNAPLT CCYSFTSKMI PMSRLESYKR ITSSRCPKEA VVFVTKLKRE VCADPKKEWV QTYIKNLDRN QMRSEPTTLF KTASALRSSA PLNVKLTRKS EANASTTFST TTSSTSVGVT SVTVN


Our Abpromise guarantee covers the use of ab9901 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies


  • Endotoxin level
    < 0.100 Eu/µg
  • Form
  • Additional notes
    The biological activity of this product was determined by its ability to chemoattract Balb/c mouse spleen MNCs using a concentration range of 0.1-10.0ng/ml.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

General Info

  • Alternative names
    • C-C motif chemokine 2
    • CCL2
    • CCL2_HUMAN
    • Chemokine (C C motif) ligand 2
    • GDCF 2
    • GDCF-2
    • GDCF2
    • HC11
    • HSMCR30
    • JE
    • MCAF
    • MCP 1
    • MCP-1
    • MCP1
    • MGC9434
    • Monocyte chemoattractant protein 1
    • Monocyte chemotactic and activating factor
    • Monocyte chemotactic protein 1
    • Monocyte secretory protein JE
    • SCYA2
    • Small inducible cytokine A2
    • Small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig je)
    • Small inducible cytokine subfamily A (Cys Cys), member 2
    • Small-inducible cytokine A2
    • SMC CF
    • SMC-CF
    • SMCCF
    see all
  • Function
    Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.
  • Sequence similarities
    Belongs to the intercrine beta (chemokine CC) family.
  • Post-translational
    Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant.
  • Cellular localization
  • Information by UniProt


This product has been referenced in:
  • Gallagher JJ  et al. Acute and chronic phases of complex regional pain syndrome in mice are accompanied by distinct transcriptional changes in the spinal cord. Mol Pain 9:40 (2013). Mouse . Read more (PubMed: 23924076) »
See 1 Publication for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Western blot
The protein ran at a slightly higher molecular weight than expected (ran around ~16kDa as opposed to the expected 13kDa). It also has fragments around ~10kDa. Literature did not indicate a cleavage site.

The protein is not readily visible in sypro at 100ng load. We visualize it in western blot with goat polyclonal antibody to MCP-1

Abcam user community

Verified customer

Submitted Sep 03 2014


The reconstitution instructions are lot-dependent. I think I tracked down your order and the lot number you have is GR54881-4, in which case the following instructions apply:

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2 to 8C for up to 1 week. For extended storage, we recommend to further dilute in a buffer containing a carrier protein (0.1% BSA) and to store in working aliquots at -20 to -80C. Avoid freeze/thaw cycles.

If you can not add BSA because you plan to use the protein in a functional study, I would recommend reconstituting in pure water, aliquotting at around 0.1 mg/mL, and freezing. If the protein is diluted further you will probably need to add BSA.

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Thank you for your enquiry.

The following antibodies from our catalog have been used with the following antibodies. they should therefore be suitable for use together. When proteins have been used with specific antibodies from the catalog, this is usually noted on the datasheet.

MCP1 protein (Active) (ab73866) has been used with MCP1 antibody [EP1361] (ab151538).
https://www.abcam.com/index.html?datasheet=73866 (or use the following: https://www.abcam.com/index.html?datasheet=73866).
https://www.abcam.com/index.html?datasheet=51538 (or use the following: https://www.abcam.com/index.html?datasheet=51538).

MCP1 protein (Active) (ab9670) has been used with MCP1 antibody (ab9669) and MCP1 antibody (ab9858)

https://www.abcam.com/index.html?datasheet=9670 (or use the following: https://www.abcam.com/index.html?datasheet=9670).
https://www.abcam.com/index.html?datasheet=9669 (or use the following: https://www.abcam.com/index.html?datasheet=9669).
https://www.abcam.com/index.html?datasheet=9858 (or use the following: https://www.abcam.com/index.html?datasheet=9858).

MCP1 protein (Active) (ab9901) has been used wtih MCP1 antibody [ECE.2] (ab8101) and MCP1 antibody (ab9899)
https://www.abcam.com/index.html?datasheet=9901 (or use the following: https://www.abcam.com/index.html?datasheet=9901).
https://www.abcam.com/index.html?datasheet=8101 (or use the following: https://www.abcam.com/index.html?datasheet=8101).
https://www.abcam.com/index.html?datasheet=9899 (or use the following: https://www.abcam.com/index.html?datasheet=9899).

The proteins are sold lyophilized, I am sorry we do not sell them reconstituted. Also, please note that these antibodies and protein are not tested for this particular application, and so will regrettably not be covered by the 6 month guarantee for this purpose.

I hope this will be helpful. If you have any further questions, please do not hesitate to contact us.

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