Recombinant mouse MDH2 protein (Active) (ab229176)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies, MS
Description
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Product name
Recombinant mouse MDH2 protein (Active)
See all MDH2 proteins and peptides -
Biological activity
Specific activity is > 800 units/mg, and is defined as the amount of enzyme that cleaves 1 µmole of oxaloacetate and beta-NADH to L-malate and beta-NAD per minute at pH8.0 at 37°C.
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Purity
> 95 % SDS-PAGE.
ab229176 was purified using conventional chromatography techniques. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Mouse -
Sequence
MGSSHHHHHHSSGLVPRGSHMAKVAVLGASGGIGQPLSLLLKNSPLVSRL TLYDIAHTPGVAADLSHIETRANVKGYLGPEQLPDCLKGCDVVVIPAGVP RKPGMTRDDLFNTNATIVATLTAACAQHCPEAMVCIIANPVNSTIPITAE VFKKHGVYNPNKIFGVTTLDIVRANTFVAELKGLDPARVNVPVIGGHAGK TIIPLISQCTPKVDFPQDQLATLTGRIQEAGTEVVKAKAGAGSATLSMAY AGARFVFSLVDAMNGKEGVVECSFVQSKETECTYFSTPLLLGKKGLEKNL GIGKITPFEEKMIAEAIPELKASIKKGEDFVKNMK -
Predicted molecular weight
35 kDa including tags -
Amino acids
25 to 338 -
Tags
His tag N-Terminus -
Additional sequence information
This product is the mature full length protein from aa 25 to 338. The transit peptide is not included (NP_032643).
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab229176 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
Mass Spectrometry
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Mass spectrometry
MALDI-TOF -
Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: PBS, 20% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- M MDH
- Malate dehydrogenase
- Malate dehydrogenase 2, NAD (mitochondrial)
see all -
Sequence similarities
Belongs to the LDH/MDH superfamily. MDH type 1 family. -
Post-translational
modificationsAcetylation is enhanced by up to 67% after treatment either with trichostin A (TSA) or with nicotinamide (NAM) with the appearance of tri-and tetraacetylations. Glucose also increases acetylation by about 60%. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab229176 has not yet been referenced specifically in any publications.