Description

  • Product name

    Recombinant Mouse Meprin beta protein (His tag)
  • Purity

    > 90 % SDS-PAGE.
    ab219235 was purified using conventional chromatography techniques.
  • Endotoxin level

    1.000 Eu/µg
  • Expression system

    Baculovirus
  • Accession

  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Mouse
    • Sequence

      LPAPEKFVKD IDGGIDQDIF DINQGLGLDL FEGDIKLEAN GKNSIIGDHK RWPHTIPYVL EDSLEMNAKG VILNAFERYR LKTCIDFKPW SGEANYISVF KGSGCWSSVG NIHAGKQELS IGTNCDRIAT VQHEFLHALG FWHEQSRADR DDYVIIVWDR IQPGKEHNFN IYNDSVSDSL NVPYDYTSVM HYSKTAFQNG TESTIVTRIS EFEDVIGQRM DFSDYDLLKL NQLYNCTSSL SFMDSCDFEL ENICGMIQSS GDSADWQRVS QVLSGPESDH SKMGQCKDSG FFMHFNTSIL NEGATAMLES RLLYPKRGFQ CLEFYLYNSG SGNDQLNIYT REYTTGQQGG VLTLQRQIKE VPIGSWQLHY VTLQVTKKFR VVFEGLRGPG TSSGGLSIDD INLSETRCPH HIWHIQNFTQ ILGGQDTSVY SPPFYSSKGY AFQIYMDLRS STNVGIYFHL ISGANDDQLQ WPCPWQQATM TLLDQNPDIR QRMFNQRSIT TDPTMTSDNG SYFWDRPSKV GVTDVFPNGT QFSRGIGYGT TVFITRERLK SREFIKGDDI YILLTVEDIS HLNSTSAVPD PVPTLAVHNA CSEVVCQNGG ICVVQDGRAE CKCPAGEDWW YMGKRCEKRG STRDVEHHHH HH
    • Predicted molecular weight

      73 kDa including tags
    • Amino acids

      21 to 654
    • Tags

      His tag C-Terminus
    • Additional sequence information

      (NP_032612)

Specifications

Our Abpromise guarantee covers the use of ab219235 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 7.4
    Constituents: 10% Glycerol, 90% PBS

General Info

  • Alternative names

    • Endopeptidase 2
    • Endopeptidase-2
    • kinase splitting membranal proteinase
    • KSMP
    • Mep1b
    • MEP1B_HUMAN
    • Meprin 1 beta subunit
    • Meprin A beta
    • Meprin A beta subunit precursor
    • Meprin A subunit beta
    • Meprin B
    • N benzoyl L tyrosyl P amino benzoic acid hydrolase beta subunit
    • N-benzoyl-L-tyrosyl-P-amino-benzoic acid hydrolase subunit beta
    • PABA peptide hydrolase
    • PPH beta
    see all
  • Function

    Membrane metallopeptidase that sheds many membrane-bound proteins. Known substrates include: FGF19, VGFA, IL1B, IL18, procollagen I and III, E-cadherin, KLK7, gastrin, ADAM10, tenascin-C. The presence of several pro-inflammatory cytokine among substrates implicate MEP1B in inflammation. It is also involved in tissue remodeling due to its capability to degrade extracellular matrix components.
  • Tissue specificity

    The major site of expression is the brush border membrane of small intestinal and kidney epithelial cells.
  • Sequence similarities

    Belongs to the peptidase M12A family.
    Contains 1 EGF-like domain.
    Contains 1 MAM domain.
    Contains 1 MATH domain.
  • Post-translational
    modifications

    N-glycosylated; contains high mannose and/or complex biantennary structures.
    O-glycosylation protect the C-terminal region from proteolytic cleavage and diminish secretion, this seems to be specific to human.
    Proteolytically activated by trypsin in the intestinal lumen and kallikrein-related peptidases in other tissues.
  • Cellular localization

    Cell membrane. Secreted. Homodimers are essentially membrane bound but may also be shed from the surface by ADAM-10 and ADAM-17.
  • Information by UniProt

Images

  • 15% SDS-PAGE analysis of ab219235 (3μg).

    MW: 70-100kDa (SDS-PAGE under reducing conditions).

References

ab219235 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab219235.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up