• Nature
  • Source
    BHK cells
  • Amino Acid Sequence
    • Species

Associated products


Our Abpromise guarantee covers the use of ab39309 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies


    Western blot

  • Form
  • Additional notes

    Mouse-MMP9 degrades denatured collagen (gelatin), and a range of extracellular matrix components in-vivo. Unlike MMP2, MMP9 is not constituitively produced by most normal cells, although the enzyme is often over expressed by transformed cells, tumor cells, and cells treated with the phorbol ester PMA. An endogenous inhibitor to MMP9, TIMP1, is often complexed with the enzyme in-vivo, but has been removed from this preparation.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    pH: 7.40
    Constituents: PBS, 50% Glycerol, 1.45% Sodium chloride

General Info

  • Alternative names
    • 82 kDa matrix metalloproteinase-9
    • 92 kDa gelatinase
    • 92 kDa type IV collagenase
    • CLG 4B
    • CLG4B
    • Collagenase Type 4 beta
    • Collagenase type IV 92 KD
    • EC
    • Gelatinase 92 KD
    • Gelatinase B
    • Gelatinase beta
    • GelatinaseB
    • GELB
    • Macrophage gelatinase
    • MANDP2
    • Matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
    • Matrix Metalloproteinase 9
    • MMP 9
    • MMP-9
    • MMP9
    • MMP9_HUMAN
    • Type V collagenase
    see all
  • Function
    May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-
    -Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.
  • Tissue specificity
    Produced by normal alveolar macrophages and granulocytes.
  • Involvement in disease
    Intervertebral disc disease
    Metaphyseal anadysplasia 2
  • Sequence similarities
    Belongs to the peptidase M10A family.
    Contains 3 fibronectin type-II domains.
    Contains 4 hemopexin repeats.
  • Domain
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.
    N- and O-glycosylated.
  • Cellular localization
    Secreted, extracellular space, extracellular matrix.
  • Information by UniProt


  • All lanes : Anti-MMP9 antibody (ab38898) at 2 µg/ml

    Lane 1 : Native human MMP9 protein (Proenzyme, monomer) (ab157344)
    Lane 2 : Recombinant Mouse MMP9 protein (ab39309)

    Lysates/proteins at 0.1 µg per lane.

    Performed under reducing conditions.

    This blot was produced using a 4-12% Bis-Tris gel under the MOPS buffer system. The gel was run at 200V for 60 minutes before being transferred onto a nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour before being incubated with anti-MMP9 antibody (ab38898; 2 microgram per mL)  overnight at 4°C. Antibody binding was detected using infrared labelled goat anti-rabbit  (green) antibody (diluted 1:20000) for 1 hour at room temperature before imaging.


This product has been referenced in:
  • Chiang HY  et al. R1R2 peptide ameliorates pulmonary fibrosis in mice through fibrocyte migration and differentiation. PLoS One 12:e0185811 (2017). Read more (PubMed: 28968441) »
  • Nighot P  et al. Matrix metalloproteinase 9-induced increase in intestinal epithelial tight junction permeability contributes to the severity of experimental DSS colitis. Am J Physiol Gastrointest Liver Physiol 309:G988-97 (2015). Read more (PubMed: 26514773) »
See all 3 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A


Product Recombinant Mouse MMP9 protein (ab39309) is a mixture of the active and the latent MMP-9 enzyme. This product may be suitable for zymography experiments; however, we have not directly tested ab39309 for gelatin zymography applications. Use of ab39309 in gelatin zymography applications is unfortunately not yet covered by our Abpromise Guarantee.

Should you wish to test ab39309 in an application not yet tested or validated by our labs, please inquire about our Abtrial Testing Program.

Read More


Product Recombinant Mouse MMP9 protein (ab39309) is a mixture of the active and the latent MMP-9 enzyme.

For additional information on MMP-9 activation, expression and protocol, please find our MMP-9 resources here: MMP-9 Resources. Read More

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