Key features and details
- Expression system: Escherichia coli
- Purity: > 95% Densitometry
- Suitable for: SDS-PAGE
Product nameRecombinant Mouse MYL9 protein
See all MYL9 proteins and peptides
Purity> 95 % Densitometry.
Expression systemEscherichia coli
Protein lengthFull length protein
Our Abpromise guarantee covers the use of ab60014 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab60014 (Mouse MYL9 full length protein) can be utilized as a substrate for the following active protein Kinases:
ab55674 (Active human Myosin light chain kinase protein fragment)
ab84799 (Active human MYLK3 full length protein)
ab84800 (Active human MYLK2 full length protein)
ab89742 (Active human DAP Kinase 2 full length protein)
ab190995 (Active human MYLK4 full length protein)
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
- 20 kDa myosin light chain
- Human 20kDa myosin light chain (MLC2) mRNA complete cds
FunctionMyosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion.
Tissue specificitySmooth muscle tissues and in some, but not all, nonmuscle cells.
Sequence similaritiesContains 3 EF-hand domains.
modificationsPhosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab60014 has not yet been referenced specifically in any publications.