Recombinant Mouse NTAL protein (ab56522)
Description
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Product name
Recombinant Mouse NTAL protein
See all NTAL proteins and peptides -
Purity
> 95 % SDS-PAGE.
Purified by Immobilized Metal Affinity Chromatography used for purification of polyhistidine-tagged recombinant proteins. ab56522 is filter sterilized prior to lyophilisation. -
Expression system
Escherichia coli -
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Mouse -
Amino acids
30 to 203
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab56522 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
SDS-PAGE
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 8.00
Constituents: 0.164% Sodium phosphate, 0.58% Sodium chloride -
ReconstitutionReconstitute with 100 ul sterile deionized water. For long term storage or for preparation of more diluted working stock solution, a carrier protein (e.g. 1% serum albumin) should be added to the vial.
General Info
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Alternative names
- HSPC046
- LAB
- LAT2
see all -
Function
Involved in FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. May also be involved in BCR (B-cell antigen receptor)-mediated signaling in B-cells and FCGR1 (high affinity immunoglobulin gamma Fc receptor I)-mediated signaling in myeloid cells. Couples activation of these receptors and their associated kinases with distal intracellular events through the recruitment of GRB2. -
Tissue specificity
Highly expressed in spleen, peripheral blood lymphocytes, and germinal centers of lymph nodes. Also expressed in placenta, lung, pancreas and small intestine. Present in B-cells, NK cells and monocytes. Absent from T-cells (at protein level). -
Involvement in disease
Note=LAT2 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of LAT2 may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease. -
Post-translational
modificationsPhosphorylated on tyrosines following cross-linking of BCR in B-cells, FCGR1 in myeloid cells, or FCER1 in mast cells; which induces the recruitment of GRB2.
May be polyubiquitinated. -
Cellular localization
Cell membrane. Present in lipid rafts. - Information by UniProt
Images
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SDS-PAGE - Recombinant Mouse NTAL protein (ab56522)SDS Page analysis of ab56522: 12% separating gel, 6% stacking gel; reducing conditions. Staining with Coomasie Blue. Lane 1 to 4: mouse NTAL recombinant protein: 2 µg, 1 µg, 0.5 µg, 0.2 µg per lane). -
Western blot - Recombinant Mouse NTAL protein (ab56522)WB analysis of ab56522: 12% separating gel, 6% stacking gel; reducing conditions. Immunostaining with NTAL specific antibody. Detection with Goat anti-mouse/ HRP secondary antibody. Lane 1 to 4: Amount of mouse NTAL recombinant protein loaded on each lane: (1) 0.050 µg, (2) 0.020 µg, (3) 0.005 µg, (4) 0.001 µg.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab56522 has not yet been referenced specifically in any publications.
