Recombinant Mouse NTAL protein (ab56522)
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Overview
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Product nameRecombinant Mouse NTAL protein
See all NTAL proteins and peptides -
Protein lengthProtein fragment
Description
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NatureRecombinant
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SourceEscherichia coli
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Amino Acid Sequence
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SpeciesMouse
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Amino acids30 to 203
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab56522 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
SDS-PAGE
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Purity> 95 % SDS-PAGE.
Purified by Immobilized Metal Affinity Chromatography used for purification of polyhistidine-tagged recombinant proteins. ab56522 is filter sterilized prior to lyophilisation. -
FormLyophilised
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 8.00
Constituents: 0.164% Sodium phosphate, 0.58% Sodium chloride -
ReconstitutionReconstitute with 100 ul sterile deionized water. For long term storage or for preparation of more diluted working stock solution, a carrier protein (e.g. 1% serum albumin) should be added to the vial.
General Info
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Alternative names
- HSPC046
- LAB
- LAT2
see all -
FunctionInvolved in FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. May also be involved in BCR (B-cell antigen receptor)-mediated signaling in B-cells and FCGR1 (high affinity immunoglobulin gamma Fc receptor I)-mediated signaling in myeloid cells. Couples activation of these receptors and their associated kinases with distal intracellular events through the recruitment of GRB2.
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Tissue specificityHighly expressed in spleen, peripheral blood lymphocytes, and germinal centers of lymph nodes. Also expressed in placenta, lung, pancreas and small intestine. Present in B-cells, NK cells and monocytes. Absent from T-cells (at protein level).
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Involvement in diseaseNote=LAT2 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of LAT2 may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.
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Post-translational
modificationsPhosphorylated on tyrosines following cross-linking of BCR in B-cells, FCGR1 in myeloid cells, or FCER1 in mast cells; which induces the recruitment of GRB2.
May be polyubiquitinated. -
Cellular localizationCell membrane. Present in lipid rafts.
- Information by UniProt
Images
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SDS-PAGE - Recombinant Mouse NTAL protein (ab56522)SDS Page analysis of ab56522: 12% separating gel, 6% stacking gel; reducing conditions. Staining with Coomasie Blue. Lane 1 to 4: mouse NTAL recombinant protein: 2 µg, 1 µg, 0.5 µg, 0.2 µg per lane). -
Western blot - Recombinant Mouse NTAL protein (ab56522)WB analysis of ab56522: 12% separating gel, 6% stacking gel; reducing conditions. Immunostaining with NTAL specific antibody. Detection with Goat anti-mouse/ HRP secondary antibody. Lane 1 to 4: Amount of mouse NTAL recombinant protein loaded on each lane: (1) 0.050 µg, (2) 0.020 µg, (3) 0.005 µg, (4) 0.001 µg.
Datasheets and documents
References
ab56522 has not yet been referenced specifically in any publications.
