Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Species
      Mouse
    • Amino acids
      30 to 203

Specifications

Our Abpromise guarantee covers the use of ab56522 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

  • Purity
    > 95 % SDS-PAGE.
    Purified by Immobilized Metal Affinity Chromatography used for purification of polyhistidine-tagged recombinant proteins. ab56522 is filter sterilized prior to lyophilisation.
  • Form
    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 100mM Sodium chloride, 10mM Sodium phosphate, pH 8

  • Reconstitution
    Reconstitute with 100 ul sterile deionized water. For long term storage or for preparation of more diluted working stock solution, a carrier protein (e.g. 1% serum albumin) should be added to the vial.

General Info

  • Alternative names
    • HSPC046
    • LAB
    • LAT2
    • Linker for activation of B cells
    • Linker for activation of B-cells
    • Linker for activation of T cells family member 2
    • Linker for activation of T cells transmembrane adaptor 2
    • Linker for activation of T-cells family member 2
    • Membrane associated adapter molecule
    • Membrane-associated adapter molecule
    • Non T cell activation linker
    • Non-T-cell activation linker
    • NTAL_HUMAN
    • WBSCR 5
    • Wbscr15
    • Williams Beuren syndrome chromosome region 15
    • Williams Beuren syndrome chromosome region 5
    • Williams-Beuren syndrome chromosomal region 15 protein
    • Williams-Beuren syndrome chromosomal region 5 protein
    • WSCR5
    see all
  • Function
    Involved in FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. May also be involved in BCR (B-cell antigen receptor)-mediated signaling in B-cells and FCGR1 (high affinity immunoglobulin gamma Fc receptor I)-mediated signaling in myeloid cells. Couples activation of these receptors and their associated kinases with distal intracellular events through the recruitment of GRB2.
  • Tissue specificity
    Highly expressed in spleen, peripheral blood lymphocytes, and germinal centers of lymph nodes. Also expressed in placenta, lung, pancreas and small intestine. Present in B-cells, NK cells and monocytes. Absent from T-cells (at protein level).
  • Involvement in disease
    Note=LAT2 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of LAT2 may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.
  • Post-translational
    modifications
    Phosphorylated on tyrosines following cross-linking of BCR in B-cells, FCGR1 in myeloid cells, or FCER1 in mast cells; which induces the recruitment of GRB2.
    May be polyubiquitinated.
  • Cellular localization
    Cell membrane. Present in lipid rafts.
  • Information by UniProt

Images

  • SDS-PAGE - NTAL protein (His tag) (ab56522)
    SDS-PAGE - NTAL protein (His tag) (ab56522)
    SDS Page analysis of ab56522: 12% separating gel, 6% stacking gel; reducing conditions. Staining with Coomasie Blue. Lane 1 to 4: mouse NTAL recombinant protein: 2 µg, 1 µg, 0.5 µg, 0.2 µg per lane).
  • Western blot - NTAL protein (His tag) (ab56522)
    Western blot - NTAL protein (His tag) (ab56522)
    WB analysis of ab56522: 12% separating gel, 6% stacking gel; reducing conditions. Immunostaining with NTAL specific antibody. Detection with Goat anti-mouse/ HRP secondary antibody. Lane 1 to 4: Amount of mouse NTAL recombinant protein loaded on each lane: (1) 0.050 µg, (2) 0.020 µg, (3) 0.005 µg, (4) 0.001 µg.

References

ab56522 has not yet been referenced specifically in any publications.

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