Bioactive grade

Recombinant mouse PCSK9 protein (ab167759)

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Description

  • Product name

    Recombinant mouse PCSK9 protein
    See all PCSK9 proteins and peptides

  • Biological activity

    Measured by its binding ability in a functional ELISA. Immobilized Human LDL receptor protein (ab220582) at 10 μg/mL (100 μL/well) can bind ab167759 with a linear range of 0.125-1 μg/mL.

    Measured by its binding ability in a BLI assay. Loaded Recombinant human LDL Receptor protein (ab220570) on Protein A Biosensor, can bind Recombinant mouse PCSK9 protein (ab167759) with an affinity constant of 2.28 nM as determined in BLI assay.

  • Purity

    > 97 % SDS-PAGE.
    ab167759 is lyophilized from 0.22 µm filtered solution.

  • Endotoxin level

    < 1.000 Eu/µg

  • Expression system

    HEK 293 cells

  • Accession

    Q80W65

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Mouse

    • Sequence

      QDEDGDYEELMLALPSQEDGLADEAAHVATATFRRCSKEAWRLPGTYIVV LMEETQRLQIEQTAHRLQTRAARRGYVIKVLHIFYDLFPGFLVKMSSDLL GLALKLPHVEYIEEDSFVFAQSIPWNLERIIPAWHQTEEDRSPDGSSQVE VYLLDTSIQGAHREIEGRVTITDFNSVPEEDGTRFHRQASKCDSHGTHLA GVVSGRDAGVAKGTSLHSLRVLNCQGKGTVSGTLIGLEFIRKSQLIQPSG PLVVLLPLAGGYSRILNAACRHLARTGVVLVAAAGNFRDDACLYSPASAP EVITVGATNAQDQPVTLGTLGTNFGRCVDLFAPGKDIIGASSDCSTCFMS QSGTSQAAAHVAGIVARMLSREPTLTLAELRQRLIHFSTKDVINMAWFPE DQQVLTPNLVATLPPSTHETGGQLLCRTVWSAHSGPTRTATATARCAPEE ELLSCSSFSRSGRRRGDWIEAIGGQQVCKALNAFGGEGVYAVARCCLVPR ANCSIHNTPAARAGLETHVHCHQKDHVLTGCSFHWEVEDLSVRRQPALRS RRQPGQCVGHQAASVYASCCHAPGLECKIKEHGISGPSEQVTVACEAGWT LTGCNVLPGASLTLGAYSVDNLCVARVHDTARADRTSGEATVAAAICCRS RPSAKASWVQ

    • Predicted molecular weight

      72 kDa including tags

    • Amino acids

      35 to 694

    • Tags

      His tag C-Terminus

    • Additional sequence information

      NCBI Accession number: AAH38085.1

Specifications

Our Abpromise guarantee covers the use of ab167759 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

    ELISA

  • Form

    Lyophilized
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 7.40
    Constituents: 94% PBS, 5% Trehalose

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Reconstitute with sterile deionized water to a concentration of 200 µg/ml.

General Info

  • Alternative names

    • Convertase subtilisin/kexin type 9 preproprotein
    • FH3
    • HCHOLA3
    • Hypercholesterolemia autosomal dominant 3
    • LDLCQ1
    • NARC 1
    • NARC-1
    • NARC1
    • Neural apoptosis regulated convertase 1
    • Neural apoptosis-regulated convertase 1
    • PC 9
    • PC9
    • PCSK 9
    • PCSK9
    • PCSK9_HUMAN
    • Proprotein convertase 9
    • Proprotein convertase PC9
    • Proprotein convertase subtilisin/kexin type 9
    • PSEC0052
    • Subtilisin/kexin like protease PC9
    • Subtilisin/kexin-like protease PC9
    see all

  • Function

    May be implicated in the differentiation of cortical neurons and may play a role in cholesterol homeostasis.

  • Tissue specificity

    Expressed in neuro-epithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells.

  • Involvement in disease

    Defects in PCSK9 are the cause of familial hypercholesterolemia 3 (FH3) [MIM:603776]. FH3 inheritance is autosomal dominant.

  • Sequence similarities

    Belongs to the peptidase S8 family.
    Contains 1 peptidase S8 domain.

  • Post-translational
    modifications

    The soluble zymogen undergoes autocatalytic intramolecular processing in the endoplasmic reticulum, resulting in the cleavage of its propeptide that remains associated with the secreted enzyme.

  • Cellular localization

    Secreted.
  • Information by UniProt

Images

  • Functional Studies - Recombinant mouse PCSK9 protein (ab167759)
    Functional Studies - Recombinant mouse PCSK9 protein (ab167759)

    Immobilized Human LDL receptor protein (ab220582) at 10 μg/mL (100 μL/well) can bind ab167759 with a linear range of 0.125-1 μg/mL.

  • SDS-PAGE - Recombinant mouse PCSK9 protein (ab167759)
    SDS-PAGE - Recombinant mouse PCSK9 protein (ab167759)

    SDS-PAGE analysis of reduced ab167759 stained overnight with Coomassie Blue. The protein migrates as 17 kDa and 65 kDa under reducing (R) condition due to glycosylation and proteolytic digestion.

  • Functional Studies - Recombinant mouse PCSK9 protein (ab167759)
    Functional Studies - Recombinant mouse PCSK9 protein (ab167759)

    Loaded Recombinant human LDL Receptor protein (ab220570) on Protein A Biosensor, can bind Recombinant mouse PCSK9 protein (ab167759) with an affinity constant of 2.28 nM as determined in BLI assay.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References

Publishing research using ab167759? Please let us know so that we can cite the reference in this datasheet.

ab167759 has been referenced in 2 publications.

  • Kawakami R  et al. Development of vaccine for dyslipidemia targeted to a proprotein convertase subtilisin/kexin type 9 (PCSK9) epitope in mice. PLoS One 13:e0191895 (2018). PubMed: 29438441
  • Sun H  et al. PCSK9 deficiency reduces atherosclerosis, apolipoprotein B secretion, and endothelial dysfunction. J Lipid Res 59:207-223 (2018). PubMed: 29180444

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