Name: Recombinant mouse PCSK9 protein
SKU: ab167759

Product name

Recombinant mouse PCSK9 protein
See all PCSK9 proteins and peptides
Protein length

Full length protein

Our Abpromise guarantee covers the use of ab167759 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Biological activity

Measured by its binding ability in a functional ELISA. Immobilized mouse PCSK9 at 10 µg/ml (100 µl/well) can bind biotinylated rhLDLR.
The EC₅₀ of biotinylated Human LDLR is 0.1 µg/ml.
Applications

Functional Studies

SDS-PAGE

ELISA
Endotoxin level

< 1.000 Eu/µg
Purity

> 97 % SDS-PAGE.
ab167759 is lyophilized from 0.22 µm filtered solution.
Form

Lyophilised
Concentration information loading...

Stability and Storage

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

pH: 7.40
Constituents: 94% PBS, 5% Trehalose

This product is an active protein and may elicit a biological response in vivo, handle with caution.
Reconstitution

It is strongly recommended to reconstitute the protein with 1000 µl PBS, pH7.4 to a stock solution of 100 µg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.

Alternative names
- Convertase subtilisin/kexin type 9 preproprotein
- FH3
- HCHOLA3
- Hypercholesterolemia autosomal dominant 3
- LDLCQ1
- NARC 1
- NARC-1
- NARC1
- Neural apoptosis regulated convertase 1
- Neural apoptosis-regulated convertase 1
- PC 9
- PC9
- PCSK 9
- PCSK9
- PCSK9_HUMAN
- Proprotein convertase 9
- Proprotein convertase PC9
- Proprotein convertase subtilisin/kexin type 9
- PSEC0052
- Subtilisin/kexin like protease PC9
- Subtilisin/kexin-like protease PC9
see all
Function

May be implicated in the differentiation of cortical neurons and may play a role in cholesterol homeostasis.
Tissue specificity

Expressed in neuro-epithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells.
Involvement in disease

Defects in PCSK9 are the cause of familial hypercholesterolemia 3 (FH3) [MIM:603776]. FH3 inheritance is autosomal dominant.
Sequence similarities

Belongs to the peptidase S8 family.
Contains 1 peptidase S8 domain.
Post-translational
modifications

The soluble zymogen undergoes autocatalytic intramolecular processing in the endoplasmic reticulum, resulting in the cleavage of its propeptide that remains associated with the secreted enzyme.
Cellular localization

Secreted.
Target information above from: UniProt accession Q8NBP7 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt

SDS-PAGE - Recombinant mouse PCSK9 protein (ab167759)

SDS-PAGE analysis of ab167759 stained overnight with Coomassie Blue.

Kawakami R et al. Development of vaccine for dyslipidemia targeted to a proprotein convertase subtilisin/kexin type 9 (PCSK9) epitope in mice. PLoS One 13:e0191895 (2018). Read more (PubMed: 29438441) »

Publishing research using ab167759? Please let us know so that we can cite the reference in this datasheet.

Recombinant mouse PCSK9 protein (ab167759)

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