Recombinant mouse PD-L1 protein (Fc Chimera Active) (ab221310)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: Fc tag C-Terminus
- Suitable for: SDS-PAGE, ELISA
Description
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Product name
Recombinant mouse PD-L1 protein (Fc Chimera Active)
See all PD-L1 proteins and peptides -
Biological activity
Measured by its binding ability in a functional ELISA. Immobilized ab221310 at 5 μg/mL (100 μL/well) can bind Recombinant mouse PD1 protein (Active) (Biotin) (ab246151) with a linear range of 0.313-5 μg/mL.
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Mouse CD80 protein (Fc Chimera) (ab207101) at 5 μg/mL (100 μL/well) can bind ab221310 with a linear range of 0.16-2.5 μg/mL.
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Purity
> 95 % SDS-PAGE. -
Endotoxin level
< 1.000 Eu/µg -
Expression system
HEK 293 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Mouse -
Sequence
FT ITAPKDLYVV EYGSNVTMEC RFPVERELDL LALVVYWEKE DEQVIQFVAG EEDLKPQHSN FRGRASLPKD QLLKGNAALQ ITDVKLQDAG VYCCIISYGG ADYKRITLKV NAPYRKINQR ISVDPATSEH ELICQAEGYP EAEVIWTNSD HQPVSGKRSV TTSRTEGMLL NVTSSLRVNA TANDVFYCTF WRSQPGQNHT AELIIPELPA THPPQNRT -
Predicted molecular weight
52 kDa including tags -
Amino acids
19 to 238 -
Tags
Fc tag C-Terminus -
Additional sequence information
This protein carries a mouse IgG2a Fc tag (Glu 98-Lys 330; P01863) at the C-terminus. (NP_068693).
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Specifications
Our Abpromise guarantee covers the use of ab221310 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
ELISA
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Form
Lyophilized -
Additional notes
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Please see notes section.
pH: 7.40
Constituents: 95% PBS, 5% Trehalose
Lyophilized from 0.22 µm filtered solution.This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with sterile deionized water to a concentration of 400 µg/ml.
General Info
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Alternative names
- B7 H
- B7 H1
- B7 homolog 1
see all -
Function
Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production. -
Tissue specificity
Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes. -
Sequence similarities
Belongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Cell membrane and Endomembrane system. - Information by UniProt
Images
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Immobilized Recombinant Mouse CD80 protein (Fc Chimera) (ab207101) at 5 μg/mL (100 μL/well) can bind ab221310 with a linear range of 0.16-2.5 μg/mL.
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Immobilized ab221310 at 5 μg/mL (100 μL/well) can bind Recombinant mouse PD1 protein (Active) (Biotin) (ab246151) with a linear range of 0.313-5 μg/mL.
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SDS-PAGE analysis of reduced ab221310 stained overnight with Coomassie Blue.
The protein migrates as 65-85 kDa on a SDS-PAGE gel under reducing conditions due to glycosylation.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab221310 has not yet been referenced specifically in any publications.