Recombinant mouse PD1 protein (Active) (ab180051)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 90% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Description
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Product name
Recombinant mouse PD1 protein (Active)
See all PD1 proteins and peptides -
Biological activity
Loaded mouse PD-L1, Fc Tag on Protein A Biosensor, can bind ab180051 with an affinity constant of 6.9 μM as determined in BLI assay.
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Purity
> 90 % SDS-PAGE. -
Endotoxin level
< 1.000 Eu/µg -
Expression system
HEK 293 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Mouse -
Sequence
LEVPNGPWRS LTFYPAWLTV SEGANATFTC SLSNWSEDLM LNWNRLSPSN QTEKQAAFCN GLSQPVQDAR FQIIQLPNRH DFHMNILDTR RNDSGIYLCG AISLHPKAKI EESPGAELVV TERILETSTR YPSPSPKPEG RFQ -
Predicted molecular weight
17 kDa including tags -
Amino acids
25 to 167 -
Tags
His tag C-Terminus -
Additional sequence information
(NP_032824)
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab180051 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
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Form
Lyophilized -
Additional notes
Endotoxin Levels determined by LAL method.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at 4°C (stable for up to 12 months). Store at -20°C or -80°C. Avoid freeze / thaw cycle. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).
pH: 7.40
Constituents: 95% PBS, 5% Trehalose
Lyophilized from 0.22 µm filtered solution.This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with sterile deionized water to a concentration of 400 µg/ml.
General Info
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Alternative names
- CD279
- CD279 antigen
- hPD 1
see all -
Function
Possible cell death inducer, in association with other factors. -
Involvement in disease
Genetic variation in PDCD1 is associated with susceptibility to systemic lupus erythematosus type 2 (SLEB2) [MIM:605218]. Systemic lupus erythematosus is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Developmental stage
Induced at programmed cell death. -
Cellular localization
Membrane. - Information by UniProt
Images
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Reduced ab180051 on SDS-PAGE, stained overnight with Coomassie Blue.
The protein migrates as 25-45 kDa under reducing condition due to glycosylation.
Purity of protein >90%.
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Loaded Mouse PD-L1, Fc Tag on Protein A Biosensor, can bind Mouse PD-1, His Tag (ab180051) with an affinity constant of 6.9 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (1)
ab180051 has been referenced in 1 publication.
- Mitchell LA et al. PD-L1 checkpoint blockade delivered by retroviral replicating vector confers anti-tumor efficacy in murine tumor models. Oncotarget 10:2252-2269 (2019). PubMed: 31040917