Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 70% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE, WB
Product nameRecombinant mouse PDE5A/PDE5 protein
See all PDE5A/PDE5 proteins and peptides
Biological activitySpecific Activity: 4630 pmol/min/µg. Assay conditions: 10 mM Tris-HCl, pH7.4, 10 mM MgCl2, 1 mM MnCl2, 200 µM cGMP, 2.5 kU 5' nucleotidase, 37ºC, 20min.
Purity> 70 % SDS-PAGE.
Expression systemBaculovirus infected Sf9 cells
Protein lengthFull length protein
Our Abpromise guarantee covers the use of ab80330 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This product was previously labelled as PDE5A
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.0462% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.395% Tris HCl, 0.05% Tween, 50% Glycerol (glycerin, glycerine), 0.58% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- 5''-cyclic phosphodiesterase
- CGB PDE
FunctionPlays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This phosphodiesterase catalyzes the specific hydrolysis of cGMP to 5'-GMP.
Tissue specificityExpressed in aortic smooth muscle cells, heart, placenta, skeletal muscle and pancreas and, to a much lesser extent, in brain, liver and lung.
PathwayPurine metabolism; 3',5'-cyclic GMP degradation; GMP from 3',5'-cyclic GMP: step 1/1.
Sequence similaritiesBelongs to the cyclic nucleotide phosphodiesterase family.
Contains 2 GAF domains.
DomainComposed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain which contains two homologous allosteric cGMP-binding regions, A and B.
modificationsPhosphorylation is regulated by binding of cGMP to the two allosteric sites.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab80330 has not yet been referenced specifically in any publications.